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Gametogenesis of intergroup hybrids of hemiclonal frogs
Journal article   Open access   Peer reviewed

Gametogenesis of intergroup hybrids of hemiclonal frogs

MATILDE Ragghianti, STEFANIA Bucci, SILVIA Marracci, CLAUDIO Casola, GIORGIO Mancino, HANSJÜRG Hotz, GASTON-DENIS Guex, JÖRG Plötner and THOMAS Uzzell
Genetical Research, v 89(1)
Feb 2007
PMID: 17517158
url
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/E43BFD423547B9AC2AB21EA67F2DA361/S0016672307008610a.pdf/div-class-title-gametogenesis-of-intergroup-hybrids-of-hemiclonal-frogs-div.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1017/S0016672307008610View
Published, Version of Record (VoR) Open

Abstract

European water frog hybrids Rana esculenta (R. ridibunda×R. lessonae) reproduce hemiclonally, by hybridogenesis: in the germ line they exclude the genome of one parental species and produce haploid gametes with an unrecombined genome of the other parental species. In the widespread L-E population system, both sexes of hybrids (E) coexist with R. lessonae (L). They exclude the lessonae genome and produce ridibunda gametes. In the R-E system, hybrid males coexist with R. ridibunda (R); they exclude either their ridibunda or their lessonae genome and produce sperm with a lessonae or with a ridibunda genome or a mixture of both kinds of sperm. We examined 13 male offspring, 12 of which were from crosses between L-E system and R-E system frogs. All were somatically hybrid. With one exception, they excluded the lessonae genome in the germ line and subsequently endoreduplicated the ridibunda genome. Spermatogonial metaphases contained a haploid or a diploid number of ridibunda chromosomes, identified through in situ hybridization to a satellite DNA marker, and by spermatocyte I metaphases containing a haploid number of ridibunda bivalents. The exception, an F1 hybrid between L-E system R. lessonae and R-E system R. ridibunda, was not hybridogenetic, showed no genome exclusion, and evidenced a disturbed gametogenesis resulting from the combination of two heterospecific genomes. None of the hybridogenetic hybrids showed any cell lines excluding the ridibunda genome, the pattern most frequent in hybrids of the R-E system, unique to that system, and essential for its persistence. A particular combination of R-E system lessonae and R-E system ridibunda genomes seems necessary to induce the R-E system type of hemiclonal gametogenesis.

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Genetics & Heredity
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