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Journal article
Gelatin-Based Microribbon Hydrogels Accelerate Cartilage Formation by Mesenchymal Stem Cells in Three Dimensions
Tissue engineering. Part A, v 24(21-22), pp 1631-1640
01 Nov 2018
PMID: 29926770
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hydrogels (HGs) are attractive matrices for cell-based cartilage tissue regeneration given their injectability and ability to fill defects with irregular shapes. However, most HGs developed to date often lack cell scale macroporosity, which restrains the encapsulated cells, leading to delayed new extracellular matrix deposition restricted to pericellular regions. Furthermore, tissue-engineered cartilage using conventional HGs generally suffers from poor mechanical property and fails to restore the load-bearing property of articular cartilage. The goal of this study was to evaluate the potential of macroporous gelatin-based microribbon (RB) HGs as novel 3D matrices for accelerating chondrogenesis and new cartilage formation by human mesenchymal stem cells (MSCs) in 3D with improved mechanical properties. Unlike conventional HGs, these RB HGs are inherently macroporous and exhibit cartilage-mimicking shock-absorbing mechanical property. After 21 days of culture, MSC-seeded RB scaffolds exhibit a 20-fold increase in compressive modulus to 225kPa, a range that is approaching the level of native cartilage. In contrast, HGs only resulted in a modest increase in compressive modulus of 65kPa. Compared with conventional HGs, macroporous RB scaffolds significantly increased the total amount of neocartilage produced by MSCs in 3D, with improved interconnectivity and mechanical strength. Altogether, these results validate gelatin-based RBs as promising scaffolds for enhancing and accelerating MSC-based cartilage regeneration and may be used to enhance cartilage regeneration using other cell types as well.
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Details
- Title
- Gelatin-Based Microribbon Hydrogels Accelerate Cartilage Formation by Mesenchymal Stem Cells in Three Dimensions
- Creators
- Bogdan Conrad - Stanford UniversityLi-Hsin Han - Stanford UniversityFan Yang - Stanford University
- Publication Details
- Tissue engineering. Part A, v 24(21-22), pp 1631-1640
- Publisher
- Mary Ann Liebert, Inc
- Number of pages
- 10
- Grant note
- CBET-1351289 / National Science Foundation CAREER award program Stanford Child Health Research Institute California Institute for Regenerative Medicine predoctoral fellowship TR3-05569 / California Institute for Regenerative Medicine Tools and Technologies award F31DE025788 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) R01DE024772-01 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Stanford Chem-H Institute New Materials for Applications in Biology and Medicine Seed Grant 5F31DE025788-03 / NIH NRSA predoctoral fellowship; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics; Drexel University
- Web of Science ID
- WOS:000449368500006
- Scopus ID
- 2-s2.0-85056171506
- Other Identifier
- 991020100183304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical
- Materials Science, Biomaterials