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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix
Science (American Association for the Advancement of Science), v 345(6200), pp 1062-1065
29 Aug 2014
PMID: 25170155
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cells use actomyosin contractility to move through three-dimensional (3D) extracellular matrices. Contractility affects the type of protrusions cells use to migrate in 3D, but the mechanisms are unclear. In this work, we found that contractility generated high-pressure lobopodial protrusions in human cells migrating in a 3D matrix. In these cells, the nucleus physically divided the cytoplasm into forward and rear compartments. Actomyosin contractility with the nucleoskeleton-intermediate filament linker protein nesprin-3 pulled the nucleus forward and pressurized the front of the cell. Reducing expression of nesprin-3 decreased and equalized the intracellular pressure. Thus, the nucleus can act as a piston that physically compartmentalizes the cytoplasm and increases the hydrostatic pressure between the nucleus and the leading edge of the cell to drive lamellipodia-independent 3D cell migration.
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Details
- Title
- Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix
- Creators
- Ryan J Petrie - National Institute of Dental and Craniofacial ResearchHyun Koo - National Institutes of HealthKenneth M Yamada - National Institute of Dental and Craniofacial Research
- Publication Details
- Science (American Association for the Advancement of Science), v 345(6200), pp 1062-1065
- Publisher
- American Association for the Advancement of Science (AAAS)
- Grant note
- Z01 DE000524-17 / Intramural NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000340870900043
- Scopus ID
- 2-s2.0-84907266423
- Other Identifier
- 991020100087804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology