Journal article
Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection
PLoS pathogens, v 6(9), pp e1001108-e1001108
16 Sep 2010
PMID: 20862314
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Although there is tremendous interest in understanding the evolutionary roles of horizontal gene transfer (HGT) processes that occur during chronic polyclonal infections, to date there have been few studies that directly address this topic. We have characterized multiple HGT events that most likely occurred during polyclonal infection among nasopharyngeal strains of Streptococcus pneumoniae recovered from a child suffering from chronic upper respiratory and middle-ear infections. Whole genome sequencing and comparative genomics were performed on six isolates collected during symptomatic episodes over a period of seven months. From these comparisons we determined that five of the isolates were genetically highly similar and likely represented a dominant lineage. We analyzed all genic and allelic differences among all six isolates and found that all differences tended to occur within contiguous genomic blocks, suggestive of strain evolution by homologous recombination. From these analyses we identified three strains (two of which were recovered on two different occasions) that appear to have been derived sequentially, one from the next, each by multiple recombination events. We also identified a fourth strain that contains many of the genomic segments that differentiate the three highly related strains from one another, and have hypothesized that this fourth strain may have served as a donor multiple times in the evolution of the dominant strain line. The variations among the parent, daughter, and grand-daughter recombinant strains collectively cover greater than seven percent of the genome and are grouped into 23 chromosomal clusters. While capturing in vivo HGT, these data support the distributed genome hypothesis and suggest that a single competence event in pneumococci can result in the replacement of DNA at multiple non-adjacent loci.
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Details
- Title
- Generation of genic diversity among Streptococcus pneumoniae strains via horizontal gene transfer during a chronic polyclonal pediatric infection
- Creators
- N Luisa Hiller - Allegheny General Hospital, Allegheny-Singer Research Institute, Center for Genomic Sciences, Pittsburgh, Pennsylvania, United States of AmericaAzad AhmedEvan PowellDarren P MartinRory EutseyJosh EarlBenjamin JantoRobert J BoissyJustin HoggKaren BarbadoraRangarajan SampathShaun LonerganJ Christopher PostFen Z HuGarth D Ehrlich
- Publication Details
- PLoS pathogens, v 6(9), pp e1001108-e1001108
- Publisher
- Public LIbrary of Science (PLOS); United States
- Grant note
- Wellcome Trust R01 AI080935 / NIAID NIH HHS R01 DC005659 / NIDCD NIH HHS R01 DC002148 / NIDCD NIH HHS DC04173 / NIDCD NIH HHS DC02148 / NIDCD NIH HHS DC05659 / NIDCD NIH HHS AI080935 / NIAID NIH HHS R01 DC004173 / NIDCD NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000282373000029
- Scopus ID
- 2-s2.0-78149306108
- Other Identifier
- 991014878099204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology
- Parasitology
- Virology