Journal article
Generation of leukotrienes by purified human lung mast cells
The Journal of clinical investigation, v 70(4), pp 747-751
01 Oct 1982
PMID: 7119113
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Although mediator release from mast cells and basophils plays a central role in the pathogenesis of human allergic disease, biochemical studies have been restricted to rat peritoneal mast cells and basophilic leukemia cells because they could be easily purified. We have used two new techniques of cell separation to purify human lung mast cells to 98% homogeneity. Lung cell suspensions were obtained by dispersion of chopped lung tissue with proteolytic enzymes. Mast cells were then purified from the suspensions by countercurrent centrifugal elutriation and affinity chromatography. The purified mast cells released both histamine and slow-reacting substance of anaphylaxis (SRS-A) (leukotriene C and D) during stimulation with goat anti-human IgE antibody. Moreover, these preparations were able to generate significant quantities of SRS-A (32 +/- 7 x 10(-17) LTD mole-equivalents/mast cell) at all stages of purification, indicating that a secondary cell is not necessary for the antigen-induced release of SRS.
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Details
- Title
- Generation of leukotrienes by purified human lung mast cells
- Creators
- D W MacGlashan - Johns Hopkins UniversityR P Schleimer - Johns Hopkins UniversityS P Peters - Johns Hopkins UniversityE S SchulmanG K AdamsH H NewballL M Lichtenstein
- Publication Details
- The Journal of clinical investigation, v 70(4), pp 747-751
- Publisher
- American Society for Clinical Investigation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pulmonary, Critical Care, and Sleep (Medicine)
- Web of Science ID
- WOS:A1982PL02000006
- Scopus ID
- 2-s2.0-0019961251
- Other Identifier
- 991019295188604721
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- Web of Science research areas
- Medicine, Research & Experimental