Journal article
Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase zeta
Molecular cell, v 79(6), pp 1037-1050.e5
17 Sep 2020
PMID: 32882183
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
DNA double-stranded breaks (DSBs) are dangerous lesions threatening genomic stability. Fidelity of DSB repair is best achieved by recombination with a homologous template sequence. In yeast, transcript RNA was shown to template DSB repair of DNA. However, molecular pathways of RNA-driven repair processes remain obscure. Utilizing assays of RNA-DNA recombination with and without an induced DSB in yeast DNA, we characterize three forms of RNA-mediated genomic modifications: RNA- and cDNA-templated DSB repair (R-TDR and c-TDR) using an RNA transcript or a DNA copy of the RNA transcript for DSB repair, respectively, and a new mechanism of RNA-templated DNA modification (R-TDM) induced by spontaneous or mutagen-induced breaks. While c-TDR requires reverse transcriptase, translesion DNA polymerase zeta (pol zeta) plays a major role in R-TDR, and it is essential for R-TDM. This study characterizes mechanisms of RNA-DNA recombination, uncovering a role of Pol zeta in transferring genetic information from transcript RNA to DNA.
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Details
- Title
- Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase zeta
- Creators
- Chance Meers - Georgia Institute of TechnologyHavva Keskin - Georgia Institute of TechnologyGabor Banyai - Georgia Institute of TechnologyOlga Mazina - Drexel UniversityTaehwan Yang - Georgia Institute of TechnologyAlli L. Gombolay - Georgia Institute of TechnologyKuntal Mukherjee - Georgia Institute of TechnologyEfiyenia Kaparos - Georgia Institute of TechnologyGary Newnam - Georgia Institute of TechnologyAlexander Mazin - Drexel UniversityFrancesca Storici - Georgia Institute of Technology
- Publication Details
- Molecular cell, v 79(6), pp 1037-1050.e5
- Publisher
- Elsevier
- Number of pages
- 19
- Grant note
- DMS-1764406 / Southeast Center for Mathematics and Biology (NSF) CA188347; P30CA056036; GM136717 / National Institute of General Medical Sciences (NIGMS) of the NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 55108574 / Howard Hughes Medical Institute Faculty Scholar GM115927 / NIGMS of the NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 594594 / Southeast Center for Mathematics and Biology (Simons Foundation) Drexel Coulter Program Award MCB-1615335 / National Science Foundation; National Science Foundation (NSF) National Cancer Institute (NCI); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000573535400005
- Scopus ID
- 2-s2.0-85090700979
- Other Identifier
- 991019169562504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology