Journal article
Genetic Strain and Sex Differences in a Hyperoxia-Induced Mouse Model of Varying Severity of Bronchopulmonary Dysplasia
The American journal of pathology, v 189(5), pp 999-1014
01 May 2019
PMID: 30794808
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bronchopulmonary dysplasia (BPD) is a disease prevalent in preterm babies with a need for supplemental oxygen, resulting in impaired lung development and dysregulated vascularization. Epidemiologic studies have shown that males are more prone to BPD and have a delayed recovery compared with females, for reasons unknown. Herein, we tried to recapitulate mild, moderate, and severe BPD, using two different strains of mice, in males and females: CD1 (outbred) and C57BL/6 (inbred). Aside from higher body weight in the CD1 strain, there were no other gross morphologic differences with respect to alveolar development between the two strains. With respect to lung morphology after oxygen exposure, females had less injury with better preservation of alveolar chord Length and decreased alveolar protein leak and inflammatory cells in the bronchoalveolar lavage fluid. In addition, housekeeping genes, which are routinely used as loading controls, were expressed differently in males and females. In the BPD mouse model, gonadotropin-releasing hormone was increased in females compared with males. Specific miRNAs (miR-146 and miR-34a) were expressed differently in the sexes. In the severe BPD mouse model, administering miR-146 mimic to males attenuated lung damage, whereas administering miR-146 inhibitor to females increased pulmonary injury.
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Details
- Title
- Genetic Strain and Sex Differences in a Hyperoxia-Induced Mouse Model of Varying Severity of Bronchopulmonary Dysplasia
- Creators
- Sean Leary - Drexel UniversityPragnya Das - Drexel UniversityDevasena Ponnalagu - Drexel UniversityHarpreet Singh - Drexel UniversityVineet Bhandari - Drexel University
- Publication Details
- The American journal of pathology, v 189(5), pp 999-1014
- Publisher
- Elsevier
- Number of pages
- 16
- Grant note
- 16GRNT29430000 / American Heart Association 270926; 282656 / Drexel University Department of Pediatrics R01-HL133050-01 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01HL133050 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000468010900008
- Scopus ID
- 2-s2.0-85064650688
- Other Identifier
- 991019167683504721
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- Web of Science research areas
- Pathology