Journal article
Genetic polymorphisms of XRCC1, HOGG1 and MGMT and micronucleus occurrence in Chinese vinyl chloride-exposed workers
Carcinogenesis (New York), v 31(6), pp 1068-1073
01 Jun 2010
PMID: 20378691
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this study, a group of 313 workers occupationally exposed to vinyl chloride monomer (VCM) and 141 normal unexposed referents were examined for chromosomal damage using the cytokinesis-blocked micronucleus (CBMN) assay in peripheral lymphocytes. We explored the relationship between genetic polymorphisms of XRCC1 (Arg194Trp, Arg280His and Arg399Gln), MGMT(Leu84Phe) and hOGG1 (Ser326Cys) and susceptibility of chromosomal damage induced by VCM. Polymerase chain reaction-restriction fragment length polymorphism techniques were used to detect polymorphisms in XRCC1, hOGG1 and MGMT. It was found that the micronuclei (MN) frequency of exposed workers (4.86 +/- 2.80)parts per thousand was higher than that of the control group (1.22 +/- 1.24)parts per thousand (P < 0.01). Increased susceptibility to chromosomal damage as evidenced by higher MN frequency was found in workers with hOGG1 326 Ser/Cys genotype [frequency ratio (FR) = 1.21, 95% confidence interval (CI): 1.02-1.46; P < 0.05], XRCC1 194 Arg/Trp (FR = 1.12, 95% CI: 1.00-1.25; P < 0.05) and XRCC1 280 Arg/His and His/His genotypes (FR = 1.12, 95% CI 1.00-1.26, P < 0.05). Moreover, among susceptibility diplotypes, CGA/CAG carriers had more risk of MN frequency compared with individuals with wild-type CGG/CGG (FR = 1.67, 95% CI: 1.19-2.23; P < 0.05). MN frequency also increased significantly with age in the exposed group (FR = 1.13, 95% CI: 1.00-1.28; P < 0.05). Thus, CB-MN was a sensitive index of early damage among VCM-exposed workers. Genotype XRCC1 Arg194Trp, Arg280His, hOGG1 Ser326Cys, diplotype CGA/CAG and higher age may have an impact on the chromosome damage induced by VCM.
Metrics
Details
- Title
- Genetic polymorphisms of XRCC1, HOGG1 and MGMT and micronucleus occurrence in Chinese vinyl chloride-exposed workers
- Creators
- Qi Wang - Fudan UniversityFang Ji - Fudan UniversityYuan Sun - Fudan UniversityYu-Lan Qiu - Fudan UniversityWei Wang - Fudan UniversityFen Wu - Fudan UniversityWen-bin Miao - Fudan UniversityYongliang Li - University of Illinois ChicagoPaul W. Brandt-Rauf - University of Illinois ChicagoZhao-lin Xia - Fudan University
- Publication Details
- Carcinogenesis (New York), v 31(6), pp 1068-1073
- Publisher
- Oxford Univ Press
- Number of pages
- 6
- Grant note
- R01-OH04192; P30-ES09089 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA NSFC30671740 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 08GWD12; 08GW2X0402 / Shanghai Bureau of Public Health
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000278218500015
- Scopus ID
- 2-s2.0-77953935721
- Other Identifier
- 991019323667404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Oncology