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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation
Biomaterials, v 66
01 Oct 2015
PMID: 26183934
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The alteration of cellular functions by anchoring macromolecules to specified organelles may reveal a new area of therapeutic potential and clinical treatment. In this work, a unique phenotype was evoked by influencing cellular behavior through the modification of subcellular structures with genetically targetable macromolecules. These fluorogen-functionalized polymers, prepared via controlled radical polymerization, were capable of exclusively decorating actin, cytoplasmic, or nuclear compartments of living cells expressing localized fluorgen-activating proteins. The macromolecular fluorogens were optimized by establishing critical polymer architecture-biophysical property relationships which impacted binding rates, binding affinities, and the level of internalization. Specific labeling of subcellular structures was realized at nanomolar concentrations of polymer, in the absence of membrane per-meabilization or transduction domains, and fluorogen-modified polymers were found to bind to protein intact after delivery to the cytosol. Cellular motility was found to be dependent on binding of macromolecular fluorogens to actin structures causing rapid cellular ruffling without migration. (C) 2015 Elsevier Ltd. All rights reserved.
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Details
- Title
- Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation
- Creators
- Andrew J. D. Magenau - Carnegie Mellon UniversitySaumya Saurabh - Carnegie Mellon UniversitySusan K. Andreko - Carnegie Mellon UniversityCheryl A. Telmer - Carnegie Mellon UniversityBrigitte F. Schmidt - Carnegie Mellon UniversityAlan S. Waggoner - Carnegie Mellon UniversityMarcel P. Bruchez - Carnegie Mellon University
- Publication Details
- Biomaterials, v 66
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- U54GM103529 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) McWilliams Graduate Fellowship U54GM103529 / NIH, through the Technology Centers for Networks and Pathways program; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Web of Science ID
- WOS:000359328200001
- Scopus ID
- 2-s2.0-84938090599
- Other Identifier
- 991019168043004721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials