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Journal article
Genomic diversity of bacteriophages infecting Microbacterium spp
PloS one, Vol.15(6), pp.e0234636-e0234636
18 Jun 2020
PMID: 32555720
Abstract
The bacteriophage population is vast, dynamic, old, and genetically diverse. The genomics of phages that infect bacterial hosts in the phylum Actinobacteria show them to not only be diverse but also pervasively mosaic, and replete with genes of unknown function. To further explore this broad group of bacteriophages, we describe here the isolation and genomic characterization of 116 phages that infectMicrobacteriumspp. Most of the phages are lytic, and can be grouped into twelve clusters according to their overall relatedness; seven of the phages are singletons with no close relatives. Genome sizes vary from 17.3 kbp to 97.7 kbp, and their G+C% content ranges from 51.4% to 71.4%, compared to similar to 67% for theirMicrobacteriumhosts. The phages were isolated on five differentMicrobacteriumspecies, but typically do not efficiently infect strains beyond the one on which they were isolated. TheseMicrobacteriumphages contain many novel features, including very large viral genes (13.5 kbp) and unusual fusions of structural proteins, including a fusion of VIP2 toxin and a MuF-like protein into a single gene. These phages and their genetic components such as integration systems, recombineering tools, and phage-mediated delivery systems, will be useful resources for advancingMicrobacteriumgenetics.
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Details
- Title
- Genomic diversity of bacteriophages infecting Microbacterium spp
- Creators
- Deborah Jacobs-Sera - University of PittsburghLawrence A. Abad - University of PittsburghRichard M. Alvey - Illinois Wesleyan UniversityKirk R. Anders - Gonzaga UniversityHaley G. Aull - University of PittsburghSuparna S. Bhalla - Mount Saint Mary CollegeLawrence S. Blumer - Morehouse CollegeDavid W. Bollivar - Illinois Wesleyan UniversityJ. Alfred Bonilla - University of Wisconsin–River FallsKristen A. Butela - University of PittsburghRoy J. Coomans - North Carolina Agricultural and Technical State UniversitySteven G. Cresawn - James Madison UniversityTom D'Elia - Indian River State Coll, Dept Biol Sci, Ft Pierce, FL USAArturo Diaz - La Sierra UniversityAshley M. Divens - University of PittsburghNicholas P. Edgington - Southern Connecticut State UniversityGregory D. Frederick - LeTourneau UniversityMaria D. Gainey - Western Carolina UniversityRebecca A. Garlena - University of PittsburghKenneth W. Grant - Wake Forest Baptist Medical CenterSusan M. R. Gurney - Drexel UniversityHeather L. Hendrickson - Massey UniversityLee E. Hughes - University of North TexasMargaret A. Kenna - Lehigh UniversityKaren K. Klyczek - University of Wisconsin–River FallsHari Kotturi - University of Central OklahomaTravis N. Mavrich - University of PittsburghAngela L. McKinney - Nebraska Wesleyan UniversityEvan C. Merkhofer - Mount Saint Mary CollegeJordan Moberg Parker - University of California, Los AngelesSally D. Molloy - University of MaineDenise L. Monti - University of Alabama at BirminghamDana A. Pape-Zambito - University of the SciencesRichard S. Pollenz - University of South FloridaWelkin H. Pope - University of PittsburghNathan S. Reyna - Ouachita Baptist UniversityClaire A. Rinehart - Western Kentucky UniversityDaniel A. Russell - University of PittsburghChristopher D. Shaffer - University of WashingtonViknesh Sivanathan - Howard Hughes Medical InstituteTy H. Stoner - University of PittsburghJoseph Stukey - Hope CollegeC. Nicole Sunnen - University of the SciencesSara S. Tolsma - Northwestern College - IowaPhilippos K. Tsourkas - University of Nevada, Las VegasJamie R. Wallen - Western Carolina UniversityVassie C. Ware - Lehigh UniversityMarcie H. Warner - University of PittsburghJacqueline M. Washington - Nyack CollegeKristi M. Westover - Winthrop UniversityJoAnn L. Whitefleet-Smith - Worcester Polytechnic InstituteHelen Wiersma-Koch - Indian River State CollegeDaniel C. Williams - Coastal Carolina UniversityKira M. Zack - University of PittsburghGraham F. Hatfull - University of Pittsburgh
- Publication Details
- PloS one, Vol.15(6), pp.e0234636-e0234636
- Publisher
- Public Library Science
- Number of pages
- 41
- Grant note
- GM116884; GM131729 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Provost Internal Support grant from Western Carolina University 54308198; GT12053 / Howard Hughes Medical Institute
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Identifiers
- 991019167671904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology