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Genomic diversity of bacteriophages infecting Microbacterium spp
Journal article   Open access   Peer reviewed

Genomic diversity of bacteriophages infecting Microbacterium spp

Deborah Jacobs-Sera, Lawrence A. Abad, Richard M. Alvey, Kirk R. Anders, Haley G. Aull, Suparna S. Bhalla, Lawrence S. Blumer, David W. Bollivar, J. Alfred Bonilla, Kristen A. Butela, …
PloS one, v 15(6), pp e0234636-e0234636
18 Jun 2020
PMID: 32555720
url
https://doi.org/10.1371/journal.pone.0234636View
Published, Version of Record (VoR)CC BY V4.0 Open

Abstract

Multidisciplinary Sciences Science & Technology Science & Technology - Other Topics
The bacteriophage population is vast, dynamic, old, and genetically diverse. The genomics of phages that infect bacterial hosts in the phylum Actinobacteria show them to not only be diverse but also pervasively mosaic, and replete with genes of unknown function. To further explore this broad group of bacteriophages, we describe here the isolation and genomic characterization of 116 phages that infectMicrobacteriumspp. Most of the phages are lytic, and can be grouped into twelve clusters according to their overall relatedness; seven of the phages are singletons with no close relatives. Genome sizes vary from 17.3 kbp to 97.7 kbp, and their G+C% content ranges from 51.4% to 71.4%, compared to similar to 67% for theirMicrobacteriumhosts. The phages were isolated on five differentMicrobacteriumspecies, but typically do not efficiently infect strains beyond the one on which they were isolated. TheseMicrobacteriumphages contain many novel features, including very large viral genes (13.5 kbp) and unusual fusions of structural proteins, including a fusion of VIP2 toxin and a MuF-like protein into a single gene. These phages and their genetic components such as integration systems, recombineering tools, and phage-mediated delivery systems, will be useful resources for advancingMicrobacteriumgenetics.

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Microbiology
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