Files and links (2)
Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
Gentamicin differentially alters cellular metabolism of cochlear hair cells as revealed by NAD(P)H fluorescence lifetime imaging
Journal of biomedical optics, v 20(5), pp 051032-051032
17 Feb 2015
PMID: 25688541
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Aminoglycoside antibiotics are implicated as culprits of hearing loss in more than 120,000 individuals annually. Research has shown that the sensory cells, but not supporting cells, of the cochlea are readily damaged and/or lost after use of such antibiotics. High-frequency outer hair cells (OHCs) show a greater sensitivity to antibiotics than high- and low-frequency inner hair cells (IHCs). We hypothesize that variations in mitochondrial metabolism account for differences in susceptibility. Fluorescence lifetime microscopy was used to quantify changes in NAD(P)H in sensory and supporting cells from explanted murine cochleae exposed to mitochondrial uncouplers, inhibitors, and an ototoxic antibiotic, gentamicin (GM). Changes in metabolic state resulted in a redistribution of NAD(P)H between subcellular fluorescence lifetime pools. Supporting cells had a significantly longer lifetime than sensory cells. Pretreatment with GM increased NAD(P)H intensity in high-frequency sensory cells, as well as the NAD(P)H lifetime within IHCs. GM specifically increased NAD(P)H concentration in high-frequency OHCs, but not in IHCs or pillar cells. Variations in NAD(P)H intensity in response to mitochondrial toxins and GM were greatest in high-frequency OHCs. These results demonstrate that GM rapidly alters mitochondrial metabolism, differentially modulates cell metabolism, and provides evidence that GM-induced changes in metabolism are significant and greatest in high-frequency OHCs.
Metrics
Details
- Title
- Gentamicin differentially alters cellular metabolism of cochlear hair cells as revealed by NAD(P)H fluorescence lifetime imaging
- Creators
- Lyandysha V. Zholudeva - Drexel UniversityKristina G. Ward - Creighton UniversityMichael G. Nichols - Creighton UniversityHeather Jensen Smith - Creighton University
- Publication Details
- Journal of biomedical optics, v 20(5), pp 051032-051032
- Series
- Special Section on Laser Applications in Life Sciences
- Publisher
- Society of Photo-Optical Instrumentation Engineers
- Grant note
- RO3DC012109 / National Institute on Deafness and Other Communication Disorders R15GM085776 / MN P20GM103471 / National Institute of General Medical Sciences 8P20GM103471-09 / COBRE G20RR024001 / National Center for Research Resources of the National Institutes of Health
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000356241900037
- Scopus ID
- 2-s2.0-84923362945
- Other Identifier
- 991019168149704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Optics
- Radiology, Nuclear Medicine & Medical Imaging