Journal article
Geochemical, metagenomic and metaproteomic insights into trace metal utilization by methane-oxidizing microbial consortia in sulphidic marine sediments
Environmental microbiology, v 16(6), pp 1592-1611
Jun 2014
PMID: 24148160
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Microbes have obligate requirements for trace metals in metalloenzymes that catalyse important biogeochemical reactions. In anoxic methane- and sulphide-rich environments, microbes may have unique adaptations for metal acquisition and utilization because of decreased bioavailability as a result of metal sulphide precipitation. However, micronutrient cycling is largely unexplored in cold (10 degrees C) and sulphidic (>1mM sigma H2S) deep-sea methane seep ecosystems. We investigated trace metal geochemistry and microbial metal utilization in methane seeps offshore Oregon and California, USA, and report dissolved concentrations of nickel (0.5-270nM), cobalt (0.5-6nM), molybdenum (10-5600nM) and tungsten (0.3-8nM) in Hydrate Ridge sediment porewaters. Despite low levels of cobalt and tungsten, metagenomic and metaproteomic data suggest that microbial consortia catalysing anaerobic oxidation of methane (AOM) utilize both scarce micronutrients in addition to nickel and molybdenum. Genetic machinery for cobalt-containing vitamin B12 biosynthesis was present in both anaerobic methanotrophic archaea (ANME) and sulphate-reducing bacteria. Proteins affiliated with the tungsten-containing form of formylmethanofuran dehydrogenase were expressed in ANME from two seep ecosystems, the first evidence for expression of a tungstoenzyme in psychrophilic microorganisms. Overall, our data suggest that AOM consortia use specialized biochemical strategies to overcome the challenges of metal availability in sulphidic environments.
Metrics
1 Record Views
Details
- Title
- Geochemical, metagenomic and metaproteomic insights into trace metal utilization by methane-oxidizing microbial consortia in sulphidic marine sediments
- Creators
- Jennifer B. Glass - California Institute of TechnologyHang Yu - California Institute of TechnologyJoshua A. Steele - California Institute of TechnologyKatherine S. Dawson - California Institute of TechnologyShulei Sun - University of California San DiegoKaruna Chourey - Oak Ridge National LaboratoryChongle Pan - Oak Ridge National LaboratoryRobert L. Hettich - Oak Ridge National LaboratoryVictoria J. Orphan - California Institute of Technology
- Publication Details
- Environmental microbiology, v 16(6), pp 1592-1611
- Publisher
- Wiley
- Number of pages
- 20
- Grant note
- 0825791 / Division Of Ocean Sciences; Directorate For Geosciences; National Science Foundation (NSF); NSF - Directorate for Geosciences (GEO) DE-SC0004949 / U.S. Department of Energy (DOE) Stephan Schuster National Aeronautics and Space Administration Astrobiology Postdoctoral Fellowship OCE-0825791 / National Science Foundation; National Science Foundation (NSF) DE-SC0004949 / Department of Energy Division of Biological and Environmental Research National Aeronautics and Space Administration Astrobiology Institute (Penn State Astrobiology Research Center) OCE-0825791; MCB-0348492 / NSF; National Science Foundation (NSF) Gordon and Betty Moore Foundation; Gordon & Betty Moore Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Environmental Biogeochemistry
- Web of Science ID
- WOS:000337512000013
- Scopus ID
- 2-s2.0-84887529985
- Other Identifier
- 991022207048704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
Source: SDGs in the Output
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology