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Germline expression of mammalian CTF18, an evolutionarily conserved protein required for germ cell proliferation in the fly and sister chromatid cohesion in yeast
Journal article   Open access   Peer reviewed

Germline expression of mammalian CTF18, an evolutionarily conserved protein required for germ cell proliferation in the fly and sister chromatid cohesion in yeast

Karen M. Berkowitz, Klaus H. Kaestner and Thomas A. Jongens
Molecular human reproduction, v 14(3), pp 143-150
Mar 2008
PMID: 18250106
url
https://academic.oup.com/molehr/article-pdf/14/3/143/6183259/gan005.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1093/molehr/gan005View
Published, Version of Record (VoR) Open

Abstract

CTF18 CTF18-replication factor C-like complex DNA replication germ cell development germline expression
Cutlet/CTF18 encodes an evolutionarily conserved protein that is crucial for germline development in Drosophila melanogaster. Loss of function of cutlet in the fly results in a sterile phenotype due to the failure of germline stem cells to proliferate. CTF18 was first identified in Saccharomyces cerevisiae as a sister chromatid cohesion factor that is essential for the faithful transmission of chromosomes during DNA replication. We have cloned and characterized the human and mouse CTF18 orthologs of the D. melanogaster gene, cutlet. We have demonstrated that CTF18 mRNA is expressed in human and mouse testis and ovary, and that CTF18 protein is expressed throughout the male and female germline of the mouse. We suggest a unique biological role for CTF18 in mammalian germ cell development based on its mammalian germline expression, high degree of evolutionary conservation, and role in DNA replication and chromosomal stability in yeast.

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Web of Science research areas
Developmental Biology
Obstetrics & Gynecology
Reproductive Biology
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