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Journal article
Gestational Diabetes Alters the Metabolomic Profile in 2nd Trimester Amniotic Fluid in a Sex-Specific Manner
International journal of molecular sciences, v 19(9), p2696
10 Sep 2018
PMID: 30201937
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Maternal diabetes and obesity induce marked abnormalities in glucose homeostasis and insulin secretion in the fetus, and are linked to obesity, diabetes, and metabolic disease in the offspring, with specific metabolic characterization based on offspring sex. Gestational diabetes (GDM) has profound effects on the intrauterine milieu, which may reflect and/or modulate the function of the maternal⁻fetal unit. In order to characterize metabolic factors that affect offspring development, we profiled the metabolome of second trimester amniotic fluid (AF) from women who were subsequently diagnosed with gestational diabetes (GDM) using a targeted metabolomics approach, profiling 459 known biochemicals through gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) assays. Using a nested case-control study design, we identified 69 total biochemicals altered by GDM exposure, while sex-specific analysis identified 44 and 58 metabolites in male and female offspring, respectively. The most significant changes were in glucose, amino acid, glutathione, fatty acid, sphingolipid, and bile acid metabolism with specific changes identified based on the offspring sex. Targeted isotope dilution LC/MS confirmatory assays measured significant changes in docosahexaenoic acid and arachidonic acid. We conclude that the sex-specific alterations in GDM maternal⁻fetal metabolism may begin to explain the sex-specific metabolic outcomes seen in offspring exposed to GDM in utero.
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Details
- Title
- Gestational Diabetes Alters the Metabolomic Profile in 2nd Trimester Amniotic Fluid in a Sex-Specific Manner
- Creators
- Kathleen O'Neill - University of PennsylvaniaJacqueline Alexander - Children's Hospital of PhiladelphiaRikka Azuma - Children's Hospital of PhiladelphiaRui Xiao - University of PennsylvaniaNathaniel W Snyder - Drexel UniversityClementina A Mesaros - University of PennsylvaniaIan A Blair - University of PennsylvaniaSara E Pinney - Center for Research in Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
- Publication Details
- International journal of molecular sciences, v 19(9), p2696
- Publisher
- MDPI
- Grant note
- UL1TR001878 / NCATS NIH HHS P30 DK019525 / NIDDK NIH HHS K08 DK090302 / NIDDK NIH HHS 5R21-ES11675 / NIEHS NIH HHS N/A / McCabe Foundation K12 HD000849 / NICHD NIH HHS P30-DK19525 / NIDDK NIH HHS P30 ES013508 / NIEHS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- A.J. Drexel Autism Institute
- Web of Science ID
- WOS:000449988100232
- Scopus ID
- 2-s2.0-85053314263
- Other Identifier
- 991019167719304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary