Journal article
Glucose Transport in Brucella abortus
Journal of bacteriology, v 118(1)
01 Apr 1974
PMID: 4206873
Abstract
Brucella abortus
British strain 19 transported glucose with an apparent
K
m
of 0.16 mM and an apparent
V
max
of 250 nmol per min per mg of N. The only common glucose analogue transported was 2-deoxyglucose (2-DOG), with an apparent
K
i
of 0.73 mM. Alpha- or beta-methyl glucosides and 3-
O
-methylglucose were not transported. Transport was linear for 70 to 90 s, depending on the concentration of substrate used. 2-Deoxyglucose was transported as the free sugar and was not further metabolized once inside the cell. There was no glucose phosphoenolpyruvate phosphotransferase system (PEP-PTS) present, and there were no inhibitors present in
Brucella
cell-free extract that inhibited the
Escherichia coli
glucose PEP-PTS.
N
-Ethylmaleimide (NEM) and
p
-chloromercuribenzoate (
p
CMB) completely inhibited transport of glucose and 2-DOG. Glutathione, dithiothreitol, and β-mercaptoethanol reversed the effects of
p
CMB but not of NEM. A pH optimum of 7.2 and a temperature optimum of 37 to 45 C were observed for both
K
m
and
V
max
. The glucose transport system appeared to be constitutive for glucose transport in cells grown on fructose, galactose, erythritol, or glucose. The electron transfer inhibitors carbonyl cyanide,
m
-chlorophenylhydrazone, NaN
3
, 2,4-dinitrophenol, and KCN inhibited 2-DOG transport to a greater extent than did the metabolic energy inhibitors NaAsO
4
, iodoacetate, KF, and 2-heptyl-4-hydroxyquinoline-
N
-oxide. Dicyclohexylcarbodiimide, an inhibitor of membrane-bound adenosine triphosphatases, inhibited transport by 100%.
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Details
- Title
- Glucose Transport in Brucella abortus
- Creators
- Richard F. Rest - University of KansasDonald C. Robertson - University of Kansas
- Publication Details
- Journal of bacteriology, v 118(1)
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1974S481200033
- Scopus ID
- 2-s2.0-0016373055
- Other Identifier
- 991020830724804721