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Glucose oxidase multilayer modified microcantilevers for glucose measurement
Journal article   Peer reviewed

Glucose oxidase multilayer modified microcantilevers for glucose measurement

Xiaodong Yan, Xiaohe Karen Xu and Hai-Feng Ji
Analytical chemistry (Washington), v 77(19), pp 6197-6204
01 Oct 2005
PMID: 16194079

Abstract

Glucose Oxidase - chemistry Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Glucose Oxidase - metabolism Reproducibility of Results Temperature Time Factors Glucose - metabolism Protein Conformation Glucose - analysis Hydrogen Peroxide - metabolism
We report a novel enzyme-based microcantilever sensor by using layer-by-layer nanoassembly modification. A glucose oxidase (GOx) functionalized microcantilever underwent bending when it was exposed to glucose solutions. The magnitudes of bending were proportional to the concentrations of glucose. The cantilever bending was specific toward glucose, but not to other sugars such as mannose, fructose, or galactose. The flow rate effect on the cantilever bending response is also discussed. The bending mechanism was investigated, and the kinetic and thermodynamic analysis and experimental results showed that the conformational change of GOx and gluconic formation were the origin of cantilever deflection.

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Web of Science research areas
Chemistry, Analytical
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