Journal article
Glucose-responsive microgels integrated with enzyme nanocapsules for closed-loop insulin delivery
ACS nano, v 7(8), pp 6758-6766
27 Aug 2013
PMID: 23834678
Abstract
A glucose-responsive closed-loop insulin delivery system represents the ideal treatment of type 1 diabetes mellitus. In this study, we develop uniform injectable microgels for controlled glucose-responsive release of insulin. Monodisperse microgels (256 ± 18 μm), consisting of a pH-responsive chitosan matrix, enzyme nanocapsules, and recombinant human insulin, were fabricated through a one-step electrospray procedure. Glucose-specific enzymes were covalently encapsulated into the nanocapsules to improve enzymatic stability by protecting from denaturation and immunogenicity as well as to minimize loss due to diffusion from the matrix. The microgel system swelled when subjected to hyperglycemic conditions, as a result of the enzymatic conversion of glucose into gluconic acid and protonation of the chitosan network. Acting as a self-regulating valve system, microgels were adjusted to release insulin at basal release rates under normoglycemic conditions and at higher rates under hyperglycemic conditions. Finally, we demonstrated that these microgels with enzyme nanocapsules facilitate insulin release and result in a reduction of blood glucose levels in a mouse model of type 1 diabetes.
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Details
- Title
- Glucose-responsive microgels integrated with enzyme nanocapsules for closed-loop insulin delivery
- Creators
- Zhen Gu - Massachusetts Institute of TechnologyTram T Dang - Massachusetts Institute of TechnologyMinglin Ma - Massachusetts Institute of TechnologyBenjamin C Tang - Massachusetts Institute of TechnologyHao Cheng - Massachusetts Institute of TechnologyShan Jiang - Massachusetts Institute of TechnologyYizhou Dong - Massachusetts Institute of TechnologyYunlong Zhang - Massachusetts Institute of TechnologyDaniel G Anderson - Massachusetts Institute of Technology
- Publication Details
- ACS nano, v 7(8), pp 6758-6766
- Publisher
- American Chemical Society (ACS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Web of Science ID
- WOS:000323810600034
- Scopus ID
- 2-s2.0-84883208896
- Other Identifier
- 991021886374104721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology