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Journal article
Group B Streptococcus serotypes Ia and V induce differential vaginal immune responses that may contribute to long term colonization of the female reproductive tract
American journal of reproductive immunology (1989), v 83(1), pp e13199-n/a
01 Jan 2020
PMID: 31626718
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Problem Group B Streptococcus (GBS) is a common colonizer of the female genital tract at the time of pregnancy and has been associated with severe neonatal infections. Despite trials for GBS vaccines already being underway, the factors influencing vaginal GBS colonization and clearance are currently poorly understood. Method of study Within this study, we investigated the host immune responses to GBS infections in mice that affect GBS vaginal colonization and clearance. Cervicovaginal swabs were used to measure vaginal GBS persistence, and vaginal cytokine responses were measured using the BioPlex (R) system. Lymphocytes isolated from spleens were stimulated with UV-killed GBS to examine systemic cellular responses. Additional in vitro cellular experiments using human vaginal epithelial cells were also performed, examining the effect pregnancy level hormones had on GBS adhesion, invasion, and cytokine responses. Results We observed significant differences in the ability of GBS serotype V infections to persist, compared with GBS serotype Ia vaginal infections. Vaginal cytokine response examination identified temporal changes in cytokine production (IL10, IFN gamma, IL6, IL1 beta, and TNF alpha) in relation to GBS serotype and clearance or colonization. Lymphocyte proliferation assays also revealed robust cellular immune responses to GBS vaginal infections irrespective of clearance or colonization. In vitro human cellular analyses also identified that vaginal epithelial cell line cytokine production was suppressed in the presence of hormones despite no alteration in adhesion/invasion. Conclusion Here, we establish previously unknown, serotype specific, temporal immune responses which may be associated with vaginal GBS colonization or clearance in the female genital tract.
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Details
- Title
- Group B Streptococcus serotypes Ia and V induce differential vaginal immune responses that may contribute to long term colonization of the female reproductive tract
- Creators
- Emma L. Sweeney - Queensland University of TechnologyStephanie Gardiner - Queensland University of TechnologyJacob Tickner - Queensland University of TechnologyLogan Trim - Queensland University of TechnologyKenneth W. Beagley - Queensland University of TechnologyAlison J. Carey - Queensland University of Technology
- Publication Details
- American journal of reproductive immunology (1989), v 83(1), pp e13199-n/a
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- Institute of Health & Biomedical Innovation Early Career Researcher Grant APP1052464 / National Health & Medical Research Early Career Fellowship
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine; Drexel University
- Web of Science ID
- WOS:000493773500001
- Scopus ID
- 2-s2.0-85074753220
- Other Identifier
- 991020100077904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Immunology
- Reproductive Biology