Journal article
Guanine Nucleotide Regulatory Proteins, Gq and Gi1/2, Mediate Platelet‐Activating Factor‐Stimulated Phosphoinositide Metabolism in Immortalized Hippocampal Cells
Journal of neurochemistry, v 67(4), pp 1478-1484
Oct 1996
PMID: 8858930
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Abstract
: Platelet‐activating factor (PAF) may be a neuromodulator involved in neural cell differentiation, cerebral inflammation, and ischemia. The PAF receptor is a member of the G protein‐coupled receptor superfamily. In the present study, we sought to define the specific G protein(s) that mediate PAF‐stimulated phosphoinositide (PI) metabolism in an immortalized hippocampal cell line, HN33.11. PAF increased the production of 3H‐labeled inositol phosphates (IPs) with EC50 values of 1.2–1.5 nM. The effect of PAF on 3H‐IPs formation was completely blocked by the PAF antagonist BN 50739 at a concentration of 300 nM. Pertussis toxin pretreatment attenuated PAF‐stimulated 3H‐IPs production by 20–30% (p < 0.05). Consistent with a role for Gi1/2 in this response, antiserum against Gαi1/2 blocked the response to a similar degree. Pretreatment of permeabilized cells with Gαq/11 antiserum attenuated the response by 70% (p < 0.05), suggesting a role for Gq/11 in mediating the PAF response in this cell line. Stimulation with PAF increased [α‐32P]‐GTP binding to both Gαq and Gαi1/2 proteins. Moreover, specific [3H]PAF binding sites coprecipitated with Gαq and Gαi1/2 proteins. The results suggest that PAF‐stimulated PI metabolism in HN33.11 cells is mediated by both Gq and Gi1/2 proteins.
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Details
- Title
- Guanine Nucleotide Regulatory Proteins, Gq and Gi1/2, Mediate Platelet‐Activating Factor‐Stimulated Phosphoinositide Metabolism in Immortalized Hippocampal Cells
- Creators
- Leng‐Chu ShiHoau‐Yan WangJoel HorwitzEitan Friedman
- Publication Details
- Journal of neurochemistry, v 67(4), pp 1478-1484
- Publisher
- Blackwell Science Ltd; Oxford, UK
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1996VH57800018
- Scopus ID
- 2-s2.0-0029790107
- Other Identifier
- 991014878284704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences