Journal article
HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
Scientific reports, v 6(1), pp 29447-29447
07 Jul 2016
PMID: 27385120
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The innate and adaptive immune systems fail to control HCV infection in the majority of infected individuals. HCV is an ssRNA virus, which suggests a role for Toll-like receptors (TLRs) 7 and 8 in initiating the anti-viral response. Here we demonstrate that HCV genomic RNA harbours specific sequences that initiate an anti-HCV immune response through TLR7 and TLR8 in various antigen presenting cells. Conversely, HCV particles are detected by macrophages, but not by monocytes and DCs, through a TLR7/8 dependent mechanism; this leads to chloroquine sensitive production of pro-inflammatory cytokines including IL-1 beta, while the antiviral type I Interferon response is not triggered in these cells. Antibodies to DC-SIGN, a c-type lectin selectively expressed by macrophages but not pDCs or mDCs, block the production of cytokines. Novel anti-HCV vaccination strategies should target the induction of TLR7/8 stimulation in APCs in order to establish potent immune responses against HCV.
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Details
- Title
- HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
- Creators
- Yuwei Zhang - Hôpital Saint-LucMohamed El-Far - Université de MontréalFranck P. Dupuy - Hôpital Saint-LucMohamed S. Abdel-Hakeem - Hôpital Saint-LucZhong He - Université de MontréalFrancesco Andrea Procopio - Université de MontréalYu Shi - Université de MontréalElias K. Haddad - CRCHUM, Hop St Luc, Quebec City, PQ H2X 0A9, CanadaPetronela Ancuta - Université de MontréalRafick-Pierre Sekaly - Hôpital Saint-LucElias A. Said - Université de Montréal
- Publication Details
- Scientific reports, v 6(1), pp 29447-29447
- Publisher
- Springer Nature
- Number of pages
- 13
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Infectious Diseases (and HIV Medicine); Drexel University
- Web of Science ID
- WOS:000379139800001
- Scopus ID
- 2-s2.0-84978116776
- Other Identifier
- 991020099053304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Gastroenterology & Hepatology