Journal article
HIV-1 LTR activity is influenced by the differential recruitment of Sp transcription factors during monocytic differentiation
Journal of neurovirology, Vol.12
01 May 2006
Abstract
Infection of cells of the monocyte/macrophage lineage by human immunodeficiency virus type 1 (HIV-1) has been shown to be important in the pathogenesis of the disease. Viral replication in this cell lineage is, in part, mediated by interactions between the HIV-1 long terminal repeat (LTR) and a variety of host cell and viral proteins. Consistent with this logic, basal and activated LTR activity is dependent on interactions between the G/C box array of the HIV-1 LTR and the Sp family of transcription factors. The effect of monocytic differentiation on Sp factor binding and transactivation has been examined with respect to the HIV-1 LTR. Primary monocyte-derived macrophages (MDM), as well as monoblastic (U-937 and THP-1) and myelomonocytic (HL-60) cell lines were utilized in both the absence or presence of chemical differentiating agents, dimethylsulfoxide (DMSO) or phorbol myristate acetate (PMA), to model selected aspects of monocytic differentiation. The binding of Sp1, full-length Sp3, and truncated Sp3 to a high affinity HIV-1 Sp element was examined utilizing electrophoretic mobility shift (EMS) analyses. Sp1 binding increased relative to the sum of full-length and truncated Sp3 binding following PMA-induced monocytic differentiation in U-937, THP-1, and HL-60 cells. Sp binding ratios obtained with nuclear extracts from PMA-induced cell lines were also shown to correlate to those derived from studies performed with extracts derived from primary MDMs. In addition, the altered Sp binding phenotype was shown to be associated with changes in the transcriptional activation generated by the HIV-1 G/C box array.
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Details
- Title
- HIV-1 LTR activity is influenced by the differential recruitment of Sp transcription factors during monocytic differentiation
- Creators
- M NonnemacherB IrishF KrebsE KilareskiB Wigdahl
- Publication Details
- Journal of neurovirology, Vol.12
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170592304721