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HIV-1 Latency and Eradication: Past, Present and Future
Journal article   Open access   Peer reviewed

HIV-1 Latency and Eradication: Past, Present and Future

Prasun K. Datta, Rafal Kaminski, Wenhui Hu, Vanessa Pirrone, Neil T. Sullivan, Michael R. Nonnemacher, Will Dampier, Brian Wigdahl and Kamel Khalili
Current HIV research, v 14(5), pp 431-441
01 Jan 2016
PMID: 27009094
url
https://europepmc.org/articles/pmc5157928View
Accepted (AM) Open

Abstract

Immunology Infectious Diseases Life Sciences & Biomedicine Science & Technology Virology
Background: It is well established that antiretroviral therapy (ART), while highly effective in controlling HIV replication, cannot eliminate virus from the body. Therefore, the majority of HIV-1-infected individuals remain at risk for developing AIDS due to persistence of infected reservoir cells serving as a source of virus re-emergence. Several reservoirs containing replication competent HIV-1 have been identified, most notably CD4+ T cells. Cells of the myeloid lineage, which are the first line of defense against pathogens and participate in HIV dissemination into sanctuary organs, also serve as cellular reservoirs of HIV-1. In latently infected resting CD4+ T cells, the integrated copies of proviral DNA remain in a dormant state, yet possess the ability to produce replication competent virus after cellular activation. Studies have demonstrated that modification of chromatin structure plays a role in establishing persistence, in part suggesting that latency is, controlled epigenetically. Conclusion: Current efforts to eradicate HIV-1 from this cell population focus primarily on a "shock and kill" approach through cellular reactivation to trigger elimination of virus producing cells by cytolysis or host immune responses. However, studies revealed several limitations to this approach that require more investigation to assess its clinical application. Recent advances in gene editing technology prompted use of this approach for inactivating integrated proviral DNA in the genome of latently infected cells. This technology, which requires a detailed understanding of the viral genetics and robust delivery, may serve as a powerful strategy to eliminate the latent reservoir in the host leading to a sterile cure of AIDS.

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Collaboration types
Domestic collaboration
Web of Science research areas
Immunology
Infectious Diseases
Virology
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