Journal article
HIV-1 TAR miRNA protects against apoptosis by altering cellular gene expression
Retrovirology, v 6(1), 18
16 Feb 2009
PMID: 19220914
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background: RNA interference is a gene regulatory mechanism that employs small RNA molecules such as microRNA. Previous work has shown that HIV-1 produces TAR viral microRNA. Here we describe the effects of the HIV-1 TAR derived microRNA on cellular gene expression.
Results: Using a variation of standard techniques we have cloned and sequenced both the 5' and 3' arms of the TAR miRNA. We show that expression of the TAR microRNA protects infected cells from apoptosis and acts by down-regulating cellular genes involved in apoptosis. Specifically, the microRNA down-regulates ERCC1 and IER3, protecting the cell from apoptosis. Comparison to our cloned sequence reveals possible target sites for the TAR miRNA as well.
Conclusion: The TAR microRNA is expressed in all stages of the viral life cycle, can be detected in latently infected cells, and represents a mechanism wherein the virus extends the life of the infected cell for the purpose of increasing viral replication.
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Details
- Title
- HIV-1 TAR miRNA protects against apoptosis by altering cellular gene expression
- Creators
- Zachary Klase - George Washington UniversityRafael Winograd - George Washington UniversityJeremiah Davis - George Washington UniversityLawrence Carpio - George Washington UniversityRichard Hildreth - George Washington UniversityMohammad Heydarian - George Washington UniversitySidney Fu - George Washington UniversityTimothy McCaffrey - George Washington UniversityEti Meiri - Rosetta GenomicsMila Ayash-Rashkovsky - Rosetta Genom Ltd, Rehovot, IsraelShlomit Gilad - Rosetta GenomicsZwi Bentwich - Rosetta GenomicsFatah Kashanchi - George Washington University
- Publication Details
- Retrovirology, v 6(1), 18
- Publisher
- Springer Nature
- Number of pages
- 17
- Grant note
- R21AI065236 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) McCormick Grant George Washington University REF funds AI065236; AI043894 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Snyder award
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000264672200001
- Scopus ID
- 2-s2.0-62749139614
- Other Identifier
- 991021902601004721
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- Collaboration types
- International collaboration
- Web of Science research areas
- Virology