Journal article
HOP2-MND1 modulates RAD51 binding to nucleotides and DNA
Nature communications, v 5(1), pp 4198-4198
01 Jun 2014
PMID: 24943459
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The HOP2-MND1 heterodimer is required for progression of homologous recombination in eukaryotes. In vitro, HOP2-MND1 stimulates the DNA strand exchange activities of RAD51 and DMC1. We demonstrate that HOP2-MND1 induces changes in the conformation of RAD51 that profoundly alter the basic properties of RAD51. HOP2-MND1 enhances the interaction of RAD51 with nucleotide cofactors and modifies its DNA-binding specificity in a manner that stimulates DNA strand exchange. It enables RAD51 DNA strand exchange in the absence of divalent metal ions required for ATP binding and offsets the effect of the K133A mutation that disrupts ATP binding. During nucleoprotein formation HOP2-MND1 helps to load RAD51 on ssDNA restricting its dsDNA-binding and during the homology search it promotes dsDNA binding removing the inhibitory effect of ssDNA. The magnitude of the changes induced in RAD51 defines HOP2-MND1 as a 'molecular trigger' of RAD51 DNA strand exchange.
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Details
- Title
- HOP2-MND1 modulates RAD51 binding to nucleotides and DNA
- Creators
- Dmitry V. Bugreev - Drexel UniversityFei Huang - Drexel UniversityOlga M. Mazina - Drexel UniversityRoberto J. Pezza - University of Oklahoma Health Sciences CenterOleg N. Voloshin - National Institutes of HealthR. Daniel Camerini-Otero - National Institutes of HealthAlexander V. Mazin - Drexel University
- Publication Details
- Nature communications, v 5(1), pp 4198-4198
- Publisher
- Springer Nature
- Number of pages
- 10
- Grant note
- R03MH097512 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) P20GM103636 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) W.M. Keck Foundation Award National Institute of Diabetes and Digestive and Kidney Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) R56CA100839 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) CA100839; DA033981; MH097512; GM103636 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 1054-09 / Leukemia and Lymphoma Society Scholar Award; Leukemia and Lymphoma Society R03DA033981 / NATIONAL INSTITUTE ON DRUG ABUSE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA); European Commission ZIADK052033 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000338838700023
- Scopus ID
- 2-s2.0-84903185772
- Other Identifier
- 991019168881104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology