Journal article
HOTAIR forms an intricate and modular secondary structure
Molecular cell, v 58(2), pp 353-361
16 Apr 2015
PMID: 25866246
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Long noncoding RNAs (lncRNAs) have recently emerged as key players in fundamental cellular processes and diseases, but their functions are poorly understood. HOTAIR is a 2,148-nt-long lncRNA molecule involved in physiological epidermal development and in pathogenic cancer progression, where it has been demonstrated to repress tumor and metastasis suppressor genes. To gain insights into the molecular mechanisms of HOTAIR, we purified it in a stable and homogenous form in vitro, and we determined its functional secondary structure through chemical probing and phylogenetic analysis. The HOTAIR structure reveals a degree of structural organization comparable to well-folded RNAs, like the group II intron, rRNA, or lncRNA steroid receptor activator. It is composed of four independently folding modules, two of which correspond to predicted protein-binding domains. Secondary structure elements that surround protein-binding motifs are evolutionarily conserved. Our work serves as a guide for "navigating" through the lncRNA HOTAIR and ultimately for understanding its function.
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Details
- Title
- HOTAIR forms an intricate and modular secondary structure
- Creators
- Srinivas Somarowthu - Yale UniversityMichal Legiewicz - Yale UniversityIsabel Chillón - Yale UniversityMarco Marcia - Yale UniversityFei Liu - Yale UniversityAnna Marie Pyle - Yale University
- Publication Details
- Molecular cell, v 58(2), pp 353-361
- Publisher
- Elsevier
- Grant note
- R01GM50313 / NIGMS NIH HHS Howard Hughes Medical Institute R01 GM050313 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000353222900015
- Scopus ID
- 2-s2.0-84928207166
- Other Identifier
- 991020837745004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology