Journal article
HSV-1 ICP4 deleted, Us3 defective mutant d120 infection failed to induce apoptosis in NGF-differentiated PC12 cells
Journal of neurovirology, Vol.12, pp.2-2
01 May 2006
Abstract
It has been suggested that neuronal cells respond differently in many aspects to the HSV-1 infection as compared to mitotic cells. For instance, the HSV-1 infection establishes a latent infection in neuronal cells but a lytic infection in mitotic cells. HSV-1, ICP4-deleted, Us3-defected, mutant d120 has been known to induce classical apoptosis in a variety of mitotic cell lines that have been tested. Here we report that the d120 mutant virus failed to induce apoptosis in neuronal-like, nerve growth factor (NGF) differentiated PC12 cells. More strikingly, the genome of the d120 mutant remained linear inside of the nuclei for the course of the infection, with a surprisingly long half-life of 30 days. This contrasted with the half-life of linear DNA inside of the nuclei of mitotic cells which is less than 2-3 days. The failure of the mutant d120 virus to induce apoptosis in neuronal-like cells might be due to its inability to translocate the NF-kB from the cytosol to the nuclei after viral infection. Furthermore, the mutant d120 virus was able to prolong the life of neuronal-like cells in the culture flask as compared to the mock-infected NGF-PC12 cells. This was suggested since approximately 50% of d120-infected NGF-PC12 cells remained in the culture after 45 days of infection while less than 10% of mock-infected cells were left in the culture after 45 days of infection as compared to the amount of cells at the time of infection. The possible mechanisms regarding this distinct difference in the outcome of host cells (neuronal-like NGF-PC12 cells vs the mitotic Vero cells) after mutant d120 infection are discussed.
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Details
- Title
- HSV-1 ICP4 deleted, Us3 defective mutant d120 infection failed to induce apoptosis in NGF-differentiated PC12 cells
- Creators
- B AiamkitsumritT BlockN FraserYing-Hsiu Su
- Publication Details
- Journal of neurovirology, Vol.12, pp.2-2
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991021463668304721