Files and links (1)
Published, Version of Record (VoR)CC BY-NC-ND V4.0, Open
Journal article
Head-mounted microendoscopic calcium imaging in dorsal premotor cortex of behaving rhesus macaque
Cell reports (Cambridge), v 35(11), pp 109239-109239
15 Jun 2021
PMID: 34133921
Abstract
Microendoscopic calcium imaging with one-photon miniature microscopes enables unprecedented readout of neural circuit dynamics during active behavior in rodents. In this study, we describe successful application of this technology in the rhesus macaque, demonstrating plug-and-play, head-mounted recordings of cellular-resolution calcium dynamics from large populations of neurons simultaneously in bilateral dorsal premotor cortices during performance of a naturalistic motor reach task. Imaging is stable over several months, allowing us to longitudinally track individual neurons and monitor their relationship to motor behavior over time. We observe neuronal calcium dynamics selective for reach direction, which we could use to decode the animal's trial-by-trial motor behavior. This work establishes head-mounted microendoscopic calcium imaging in macaques as a powerful approach for studying the neural circuit mechanisms underlying complex and clinically relevant behaviors, and it promises to greatly advance our understanding of human brain function, as well as its dysfunction in neurological disease.
Metrics
Details
- Title
- Head-mounted microendoscopic calcium imaging in dorsal premotor cortex of behaving rhesus macaque
- Creators
- Anil Bollimunta - InscopixSamantha R Santacruz - The University of Texas at AustinRyan W Eaton - California National Primate Research CenterPei S Xu - InscopixJohn H Morrison - California National Primate Research CenterKaren A Moxon - University of California, DavisJose M Carmena - Helen Wills Neuroscience InstituteJonathan J Nassi - Inscopix
- Publication Details
- Cell reports (Cambridge), v 35(11), pp 109239-109239
- Publisher
- Elsevier
- Grant note
- R01 NS097480 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000661869600021
- Scopus ID
- 2-s2.0-85107938484
- Other Identifier
- 991019176799004721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology