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Healing quantitative trait loci in a combined cross analysis using related mouse strain crosses
Journal article   Open access   Peer reviewed

Healing quantitative trait loci in a combined cross analysis using related mouse strain crosses

J M Cheverud, H A Lawson, R Funk, J Zhou, E P Blankenhorn and E Heber-Katz
Heredity, v 108(4), pp 441-446
30 Nov 2011
DOI: https://doi.org/10.1038/hdy.2011.94
PMID: 22126848
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1038/hdy.2011.94View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze),  Open

Abstract

combined cross mouse Original quantitative trait loci tissue regeneration wound healing
Inbred mouse strains MRL and LG share the ability to fully heal ear hole punches with the full range of appropriate tissues without scarring. They also share a common ancestry, MRL being formed from a multi-strain cross with two final backcrosses to LG before being inbred by brother-sister mating. Many gene-mapping studies for healing ability have been performed using these two strains, resulting in the location of about 20 quantitative trait loci (QTLs). Here, we combine two of these crosses ( N =638), MRL/lpr × C57BL/6NTac and LG/J × SM/J, in a single combined cross analysis to increase the mapping power, decrease QTL support intervals, separate multiple QTLs and establish allelic states at individual QTL. The combined cross analysis located 11 QTLs, 6 affecting only one cross (5 LG × SM and 1 MRL × B6) and 5 affecting both crosses, approximately the number of common QTLs expected given strain SNP similarity. Amongst the five QTLs mapped in both crosses, three had significantly different genetic effects, additive in one cross and over or underdominant in the other. It is possible that allelic states at these three loci are different in SM and B6 because they lead to differences in dominance interactions with the LG and MRL alleles. QTL support intervals are 40% smaller in the combined cross analysis than in either of the single crosses. Combined cross analysis was successful in enhancing the interpretation of earlier QTL results for these strains.

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20 citations in Web of Science
17 citations in Scopus

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UN Sustainable Development Goals (SDGs)

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#3 Good Health and Well-Being

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Collaboration types
Domestic collaboration
Web of Science research areas
Ecology
Evolutionary Biology
Genetics & Heredity
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