Journal article
Helicobacter marmotae and novel Helicobacter and Campylobacter species isolated from the livers and intestines of prairie dogs
Journal of medical microbiology, v 60(9), pp 1366-1374
01 Sep 2011
PMCID: PMC3347967
PMID: 21546560
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Prairie dogs (Cynomys ludovicianus) are used to study the aetiology and prevention of gallstones because of the similarities of prairie dog and human bile gallstone composition. Epidemiological and experimental studies have suggested a connection between infection with Helicobacter species and cholesterol cholelithiasis, cholecystis and gallbladder cancer. Ten of the 34 prairie dogs in this study had positive Helicobacter species identified by PCR using Helicobacter genus-specific primers. Ten of 34 prairie dogs had positive Campylobacter species identified in the intestine by PCR with Campylobacter genus-specific primers. Six Helicobacter sp. isolates and three Campylobacter sp. isolates were identified taxonomically by 16S rRNA gene analysis. The prairie dog helicobacters fell into three clusters adjacent to Helicobacter marmotae. On the basis of 16S rRNA gene sequence analysis, three strains in two adjacent clusters were included in the species H. marmotae. Three strains were only 97.1% similar to the sequence of H. marmotae and can be considered a novel species with the provisional designation Helicobacter sp. Prairie Dog 3. The prairie dog campylobacters formed a single novel cluster and represent a novel Campylobacter sp. with the provisional designation Campylobacter sp. Prairie Dog. They branched with Campylobacter cuniculorum at 96.3% similarity and had the greatest sequence similarity to Campylobacter helveticus at 97.1% similarity. Whether H. marmotae or the novel Helicobacter sp. and Campylobacter sp. identified in prairie dogs play a role in cholesterol gallstones or hepatobiliary disease requires further studies.
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Details
- Title
- Helicobacter marmotae and novel Helicobacter and Campylobacter species isolated from the livers and intestines of prairie dogs
- Creators
- Maike Beisele - Massachusetts Institute of TechnologyZeli Shen - Massachusetts Institute of TechnologyNicola Parry - Massachusetts Institute of TechnologyMelissa Mobley - Massachusetts Institute of TechnologyNancy S. Taylor - Massachusetts Institute of TechnologyEllen Buckley - Massachusetts Institute of TechnologyMohammad Z. Abedin - Drexel UniversityFloyd E. Dewhirst - Harvard UniversityJames G. Fox - Massachusetts Institute of Technology
- Publication Details
- Journal of medical microbiology, v 60(9), pp 1366-1374
- Publisher
- SOC GENERAL MICROBIOLOGY
- Number of pages
- 9
- Grant note
- R01CA067529; R01DK052413; T32RR007036; P30ES002109; P01CA 026731; R01DK070865 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA T32RR007036 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) P30ES002109 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) T32OD010978 / OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01CA067529 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R21DK070865 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000295018700020
- Scopus ID
- 2-s2.0-80051721524
- Other Identifier
- 991019318943604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology