Journal article
Herb pair of Ephedrae Herba-Armeniacae Semen Amarum alleviates airway injury in asthmatic rats
Journal of ethnopharmacology, v 269, 113745
06 Apr 2021
PMID: 33359859
Abstract
Ephedrae Herba (EH, Ephedra sinica Stapf.) and Armeniacae Semen Amarum (ASA, Prunus armeniaca L. var. ansu Maxim.) have been used to treat asthma, cold, fever, and cough in China for thousands of years.
In this study, we aimed to investigate the optimal ratio of EH and ASA compatibility (EAC) to reduce airway injury in asthmatic rats and its possible mechanism.
Rats were sensitized with a mixture of acetylcholine chloride and histamine bisphosphate 1 h before sensitization by intragastric administration of EAC or dexamethasone or saline for 7 days. Subsequently, the ultrastructure of rat airway epithelial tissue changes, apoptosis of the airway epithelial cells, and the expression of mRNA and protein of EGRF and Bcl-2 were detected.
Transmission electron microscope: EAC (groups C and E) had the most prominent effect on repairing airway epithelial cells' ultrastructural changes in asthmatic rats. TUNEL: dexamethasone and EAC (groups B、C、E and F) inhibited the apoptosis of airway epithelial cells in asthmatic rats (P < 0.05). In situ hybridization: EAC (group E) inhibited the overexpression of EGFR and Bcl-2 mRNA (P < 0.05).Western Blotting: EAC (groups A、B、C、E and F) inhibited the upregulation of airway epithelial EGFR and Bcl-2 protein expression (P < 0.01).
Our findings indicate that EAC can inhibit abnormal changes in airway epithelial structure and apoptosis of airway epithelial cells, thereby alleviating airway injury. In this study, the best combination of EH and ASA to alleviate airway epithelial injury in asthmatic rats was group E (EH: ASA = 8: 4.5).
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Details
- Title
- Herb pair of Ephedrae Herba-Armeniacae Semen Amarum alleviates airway injury in asthmatic rats
- Creators
- Jia-xin Ma - Jiangxi University of Traditional Chinese MedicineXiong Xiao - Jiangxi University of Traditional Chinese MedicineKai-fang Zhou - School of Pharmacy, Sanquan Medical College, Xinxiang, Henan, 453003, ChinaGang Huang - Quanzhou Normal UniversityBo Ao - Huizhou UniversityYing Zhang - Jiangxi University of Traditional Chinese MedicineWen-jun Gao - Jiangxi University of Traditional Chinese MedicineTing Lei - Jiangxi University of Traditional Chinese MedicineLi Yang - Ninth Hospital of NanchangXue-cheng Fan - Jiangxi University of Traditional Chinese MedicineWen-hong Li - Jiangxi University of Traditional Chinese Medicine
- Publication Details
- Journal of ethnopharmacology, v 269, 113745
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; College of Medicine; Drexel University
- Web of Science ID
- WOS:000613996800007
- Scopus ID
- 2-s2.0-85098626778
- Other Identifier
- 991020100081104721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Medicinal
- Integrative & Complementary Medicine
- Pharmacology & Pharmacy
- Plant Sciences