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Journal article
Herpes Simplex Virus Latency in Isolated Human Neurons
Proceedings of the National Academy of Sciences - PNAS, v 81(19), pp 6217-6221
01 Oct 1984
PMID: 6091142
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Abstract
Herpes simplex virus is most probably maintained in the ganglion neurons of the peripheral nervous system of humans in a latent form that can reactivate to produce recurrent disease. As an approximation of this cell-virus interaction, we have constructed a herpes simplex virus latency in vitro model system using human fetus sensory neurons as the host cell. Human fetus neurons were characterized as neuronal in origin by the detection of the neuropeptide substance P and the neuron-specific plasma membrane A2B5 antigen. Virus latency was established by blocking complete expression of the virus genome by treatment of infected human neurons with a combination of human leukocyte interferon and (E)-5-(2-bromovinyl)-2′-deoxyuridine for 7 days. After removal of inhibitors, virus latency was maintained for at least 9 days. This in vitro model will provide a system to analyze, in a primary human neuron, the state of the herpes simplex virus genome during establishment and maintenance of experimental latency.
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Details
- Title
- Herpes Simplex Virus Latency in Isolated Human Neurons
- Creators
- Brian WigdahlCarol A. SmithHelen M. TragliaFred Rapp
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 81(19), pp 6217-6221
- Publisher
- National Academy of Sciences of the United States of America
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1984TP31700064
- Scopus ID
- 2-s2.0-0021674538
- Other Identifier
- 991020111091204721
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