Journal article
Herpes simplex virus latency and reactivation in isolated rat sensory neurons
Virology (New York, N.Y.), v 127(1), pp 159-167
1983
PMID: 6305013
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
An in vitro herpes simplex virus type 1 (HSV-1) latency model has been established using neurons isolated from dissociated rat fetus sensory ganglia as the host cell. Rat fetal neuron cells were pretreated for 24 hr at 37° with (
E)-5-(2-bromovinyl)-2′-deoxy-uridine and human leukocyte interferon, infected with HSV-1 (approximately 2.5 plaque-forming units/cell), and treated for 7 days with the same inhibitor combination. Infectious HSV-1 became undetectable 3 days postinfection and remained undetectable during the remainder of the inhibitor treatment. After removal of inhibitors on day 7, infectious virus remained undetectable for 2–7 days; subsequently, virus replication ensued and neuronal cells were destroyed. Incubation of inhibitor-treated, infected neuron cells at 40.5° after removal of inhibitors resulted in extension of the latent period to at least 15 days. HSV-1 was reactivated from latently infected neurons by reducing the incubation temperature from 40.5 to 37° and virus-specific cytopathology was observed in neurons within 96 hr after reducing temperature. This
in vitro model system will provide the first system to analyze, in a primary cell type of neuronal origin, the state of the HSV genome during establishment and maintenance of the latent state and during virus reactivation.
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Details
- Title
- Herpes simplex virus latency and reactivation in isolated rat sensory neurons
- Creators
- Brian L. Wigdahl - Pennsylvania State UniversityRichard J. Ziegler - University of MinnesotaMary Sneve - University of MinnesotaFred Rapp
- Publication Details
- Virology (New York, N.Y.), v 127(1), pp 159-167
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1983QR80800016
- Scopus ID
- 2-s2.0-0020546115
- Other Identifier
- 991020111342504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology