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Heterogeneous response of cerebral blood flow to hypotension demonstrated by laser speckle imaging flowmetry in rats
Journal article   Peer reviewed

Heterogeneous response of cerebral blood flow to hypotension demonstrated by laser speckle imaging flowmetry in rats

Alexander Kharlamov, Benjamin R. Brown, Kirk A. Easley and Stephen C. Jones
Neuroscience letters, v 368(2), pp 151-156
2004
PMID: 15351439

Abstract

CBF-pressure autoregulation Cerebral blood flow Hemorrhagic hypotension Laser Doppler flowmetry Laser speckle imaging Lower limit
The response of cerebral blood flow (CBF) to mild hypotension shows great variability. CBF changes in different cortical regions at a mean arterial blood pressure (MABP) of 70 mmHg (near the lower limit of CBF-pressure autoregulation) were analyzed using laser speckle imaging flowmetry (LSIF). We hypothesize that variations in CBF autoregulation might be due to differences between regions within the cortex of each animal, regional heterogeneity, as opposed to differences between animals. Different responses of local CBF to hypotension in each rat were demonstrated as %CBF 70, the ratio of CBF at a MABP of 70 mmHg to CBF at a MABP of 100 mmHg. Regional heterogeneity ranged from 12 to 51%, expressed as the coefficient of variation of %CBF 70. At the same time LSIF revealed distinctly different patterns of CBF-pressure autoregulation between animals. Between-animal heterogeneity represents a continuous distribution, spread between animals with predominantly decreased CBF (%CBF 70 < 85%, rat #3) and animals with a high proportion of increased flow responses (%CBF 70 > 115%, rat #2). There are approximately equal contributions to the heterogeneity of %CBF 70 from within-animal (57 ± 5%, percent variance ± standard error) and between-animal (43 ± 26%) variations. Within-animal variations could be due to heterogeneity of vascular anatomy, while the diversity of vascular control mechanisms might contribute to between-animal variations.

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