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Heterotrophic nitrogen removal by Providencia rettgeri strain YL
Journal article   Peer reviewed

Heterotrophic nitrogen removal by Providencia rettgeri strain YL

Bin Zhao, Yi Liang He, Jue Huang, Shauna Taylor and Joseph Hughes
Journal of industrial microbiology & biotechnology, v 37(6), pp 609-616
01 Jun 2010
PMID: 20333440
url
https://doi.org/10.1007/s10295-010-0708-7View
Published, Version of Record (VoR) Restricted

Abstract

Biotechnology & Applied Microbiology Life Sciences & Biomedicine Science & Technology
Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth and nitrogen removal efficiency. A quantitative detection of nitrogen gas by gas chromatography was conducted to evaluate the nitrogen removal by strain YL. From the nitrogen balance during heterotrophic growth with 180 mg/l of NH(4) (+)-N, 44.5% of NH(4) (+)-N was in the form of N(2) and 49.7% was found in biomass, with only a trace amount of either nitrite or nitrate. The utilization of nitrite and nitrate during the ammonium removal process demonstrated that the nitrogen removal pathway by strain YL was heterotrophic nitrification-aerobic denitrification. A further enzyme assay of nitrate reductase and nitrite reductase activity under the aerobic condition confirmed this nitrogen removal pathway.

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Web of Science research areas
Biotechnology & Applied Microbiology
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