Journal article
High-Content Analysis of Proapoptotic EphA4 Dependence Receptor Functions Using Small-Molecule Libraries
Journal of biomolecular screening, v 17(6), pp 785-795
01 Jul 2012
PMID: 22492230
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Small-molecule compounds (SMCs) can provide an inexpensive and selective approach to modifying biological responses. High-content analysis (HCA) of SMC libraries can help identify candidate molecules that inhibit or activate cellular responses. In particular, regulation of cell death has important implications for many pathological conditions. Dependence receptors are a new classification of proapoptotic membrane receptors that, unlike classic death receptors, initiate apoptotic signals in the absence of their ligands. EphA4 has recently been identified as a dependence receptor that may have important functions in conditions as disparate as cancer biology and CNS injury and disease. To screen potential candidate SMCs that inhibit or activate EphA4-induced cell death, HCA of an SMC library was performed using stable EphA4-expressing NIH 3T3 cells. Our results describe a high-content method for screening dependence receptor-signaling pathways and demonstrate that several candidate SMCs can inhibit EphA4-mediated cell death.
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Details
- Title
- High-Content Analysis of Proapoptotic EphA4 Dependence Receptor Functions Using Small-Molecule Libraries
- Creators
- Claudiu M. Nelersa - University of MiamiHenry Barreras - University of MiamiErik Runko - University of MiamiJerome Ricard - University of MiamiYan Shi - University of MiamiStephanie J. Glass - University of MiamiJohn L. Bixby - University of MiamiVance P. Lemmon - University of MiamiDaniel J. Liebl - University of Miami
- Publication Details
- Journal of biomolecular screening, v 17(6), pp 785-795
- Publisher
- Sage
- Number of pages
- 11
- Grant note
- R01NS059866 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) North Dade Medical Foundation R01HD057632 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) W81XWH-05-1-0061 / DOD; United States Department of Defense Walter G. Ross Foundation Christopher and Dana Reeve Foundation T32 NS007459 / NINDS/NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) NS049545; NS059866; HD057632 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Miami Project to Cure Paralysis
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000305246000008
- Scopus ID
- 2-s2.0-84862157748
- Other Identifier
- 991021229983104721
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- Web of Science research areas
- Biochemical Research Methods
- Biotechnology & Applied Microbiology
- Chemistry, Analytical