Journal article
High-Fat Diet During the Perinatal Period Induces Loss of Myenteric Nitrergic Neurons and Increases Enteric Glial Density, Prior to the Development of Obesity
Neuroscience, v 393, pp 369-380
21 Nov 2018
PMID: 30454864
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Diet-induced obesity induces peripheral inflammation accompanied by a loss of myenteric neurons. Few studies, however, have investigated the effects of a high-fat diet (HFD) on either the development of myenteric neurons or prior to the occurrence of obesity. The present study assessed the effects of maternal HFD on the density and neurochemical phenotype of myenteric ganglia in the upper gastrointestinal tract. Sprague-Dawley rats were fed either a control or HFD (14% or 60% kcal from fat, respectively) from embryonic day 13; the fundus, corpus and duodenum were fixed thereafter at postnatal 2, 4, 6 and 12 weeks of age for subsequent immunohistochemical studies. While myenteric ganglion size did not differ throughout the study, HFD exposure decreased the number of nitrergic neurons by 6 weeks of age in all regions. This decrease was accompanied by a loss of PGP-immunoreactive neurons, suggesting a decline in myenteric neuronal number. HFD also increased myenteric plexus glial cell density in all regions by 4 weeks of age. These changes occurred in the absence of an increase in serum or gastric inflammatory markers. The present study suggests that exposure to a HFD during the perinatal time period results in glial proliferation and loss of inhibitory nitrergic neurons prior to the onset of obesity, suggesting that dietary alterations may affect gastrointestinal functions independently of increased adiposity or glycemic dysregulation.
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Details
- Title
- High-Fat Diet During the Perinatal Period Induces Loss of Myenteric Nitrergic Neurons and Increases Enteric Glial Density, Prior to the Development of Obesity
- Creators
- Caitlin A McMenamin - Pennsylvania State UniversityCourtney Clyburn - Penn State Milton S. Hershey Medical CenterCaitlin A Howe - Drexel University, Neurobiology and AnatomyKirsteen N Browning - Penn State Milton S. Hershey Medical Center
- Publication Details
- Neuroscience, v 393, pp 369-380
- Publisher
- Elsevier
- Grant note
- TL1 TR002016 / NCATS NIH HHS R01 DK111667 / NIDDK NIH HHS UL1 TR002014 / NCATS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000450777900030
- Scopus ID
- 2-s2.0-85054659406
- Other Identifier
- 991022058836804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences