Journal article
High affinity binding of Vpr to the HIV-1 LTR at C/EBP site I is correlated with HAD
Journal of neurovirology, Vol.13, pp.79-79
01 Jan 2007
Abstract
Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) is a virion-associated protein that transactivates the HIV-1 long terminal repeat (LTR). The electrophoretic mobility shift assay has been utilized to demonstrate the direct binding of purified Vpr (strain pNL4-3) to HIV-1 LTR sequences that span the adjacent CCAAT/enhancer binding protein (C/EBP) site I, NF-kappa B site II and ATF/CREB binding site (nt -95 to -130, relative to the start of transcription). Binding between HIV-1 Vpr and the LTR C/EBP site II (nt -167 to -175) has also been observed. A total of 94.7% of LTRs derived from peripheral blood displayed high relative Vpr binding affinity at C/EBP site I, while only 5.3% exhibited a low relative Vpr binding affinity phenotype. All LTRs derived from peripheral blood exhibited a high relative Vpr binding phenotype at C/EBP site II. These results suggest a preference for the maintenance of two cis-acting elements with high affinity for Vpr within LTRs derived from peripheral blood in late stage disease and during development of HIVD. Additional studies have also demonstrated that naturally occurring sequence variation within C/EBP site I and II that correlates with disease progression can dramatically alter the relative affinity of Vpr for these cis-acting elements. Further studies have also suggested a competitive interaction between Vpr and C/EBP factors for binding sites I and II. These studies indicate that Vpr may regulate the interaction of members of the C/EBP transcription factor family with the viral LTR.
Metrics
9 Record Views
Details
- Title
- High affinity binding of Vpr to the HIV-1 LTR at C/EBP site I is correlated with HAD
- Creators
- A FerrucciE KilareskiY LiuS WebsterM NonnemacherB Wigdahl
- Publication Details
- Journal of neurovirology, Vol.13, pp.79-79
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170129504721