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Accepted (AM)Open Access (License Unspecified), Open
Journal article
High antibody and cellular responses induced to HIV-1 clade C envelope following DNA vaccines delivered by electroporation
Vaccine, v 29(39), pp 6763-6770
2011
PMID: 21195801
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Clade C is the predominant HIV-1 strain infecting people in sub-Saharan Africa, India, and China and there is a critical need for a vaccine targeted to these areas. In this study we tested a DNA based vaccine that encodes the SIVgag, SIVpol and HIV-1 envelope clade C.
Rhesus macaques were immunized by electroporation with the DNA plasmid encoding optimized SIVgag, SIVpol and an HIV-1 env clade C with or without the adjuvant RANTES. Animals were monitored for immune responses and challenged following the final immunization with 25 animal infectious doses (AID) of SHIV-1157ipd3N4.
We found that the vaccine induced high levels of antigen specific IFN-γ producing effector cells and the capacity for CD4+ and CD8+ to proliferate upon antigen stimulation. Importantly, we found that the vaccine induced antibody titers as high as 1/4000. These antibodies were capable of neutralizing tier 1 HIV-1 viruses. Finally, when macaques were challenged with SHIV, viral loads were controlled in vaccinated groups.
We conclude that immunization with a simian/human immunodeficiency virus DNA-based vaccine delivered by electroporation can induce cellular and humoral immune responses that are able to control viral replication.
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Details
- Title
- High antibody and cellular responses induced to HIV-1 clade C envelope following DNA vaccines delivered by electroporation
- Creators
- Jiangmei Yin - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 SCL, Philadelphia, PA 19104, United StatesAnlan Dai - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 SCL, Philadelphia, PA 19104, United StatesJonathan LeCureux - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 SCL, Philadelphia, PA 19104, United StatesTatiana Arango - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 SCL, Philadelphia, PA 19104, United StatesMichele A Kutzler - Drexel University, College of Medicine, 245N 15th Street, Philadelphia, PA 19102, United StatesJian Yan - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 SCL, Philadelphia, PA 19104, United StatesMark G Lewis - Research Section, Bioqual, Rockville, MD 20850, United StatesAmir Khan - Inovio Pharmaceuticals, 1787 Sentry Parkway West, Blue Bell, PA 19422, United StatesNiranjan Y Sardesai - Inovio Pharmaceuticals, 1787 Sentry Parkway West, Blue Bell, PA 19422, United StatesDavid Montefiore - Duke University, Durham, NC 27710, United StatesRuth Ruprecht - Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, United StatesDavid B Weiner - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 SCL, Philadelphia, PA 19104, United StatesJean D Boyer - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 SCL, Philadelphia, PA 19104, United States
- Publication Details
- Vaccine, v 29(39), pp 6763-6770
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000295497200007
- Scopus ID
- 2-s2.0-80052285787
- Other Identifier
- 991014877813404721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Immunology
- Medicine, Research & Experimental