Journal article
High concentrations of H2O2 trigger hypertrophic cascade and phosphatase and tensin homologue (PTEN) glutathionylation in H9c2 cardiomyocytes
Experimental and molecular pathology, v 100(1), pp 199-206
Feb 2016
PMID: 26772165
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cardiac hypertrophy occurs in response to different stimuli and is mainly characterized by an enlargement of cardiomyocyte size. During hypertrophy, cardiomyocytes undergo not only radical changes of the cellular architecture but also activation of signaling cascades that counteract the atrophy genes. Experimental studies highlighted that chronic low concentrations of H2O2, induce a hypertrophic phenotype, while higher levels of H2O2 promote apoptosis.
In this study, we explored the early and long-term hypertrophic effects of high concentrations of H2O2 on H9c2 rat cardiomyocytes.
We found that 2-h stimulation with 200μM H2O2 caused an early dramatic reduction of cell viability, accompanied, 5-days later, by increased cell size and up-regulation of atrial natriuretic peptide transcription. This hypertrophic phenotype is associated to increased Akt phosphorylation and a consequent reduction of the FOXO3a and atrogin-1 gene expression. Moreover, we observed that H2O2 caused the overexpression of miR-212/miR-132 cluster concomitantly to a down-regulation of PTEN transcript without changes in its protein expression. Noteworthy, we found that the treatment of cardiomyocytes with H2O2 further led to an increase of oxidized glutathione and glutathionylation of proteins, including PTEN.
In conclusion, our results permit to reconstruct the molecular cascade triggering the cardiomyocyte hypertrophy upon high concentrations of H2O2.
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Details
- Title
- High concentrations of H2O2 trigger hypertrophic cascade and phosphatase and tensin homologue (PTEN) glutathionylation in H9c2 cardiomyocytes
- Creators
- Nadia Panera - Bambino Gesù Children's HospitalDaniela Gnani - Bambino Gesù Children's HospitalEmanuela Piermarini - Bambino Gesù Children's HospitalStefania Petrini - Bambino Gesù Children's HospitalEnrico Bertini - Bambino Gesù Children's HospitalValerio Nobili - Bambino Gesù Children's HospitalAnna Pastore - Bambino Gesù Children's HospitalFiorella Piemonte - Bambino Gesù Children's HospitalAnna Alisi - Bambino Gesù Children's Hospital
- Publication Details
- Experimental and molecular pathology, v 100(1), pp 199-206
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000369879100031
- Scopus ID
- 2-s2.0-84954323099
- Other Identifier
- 991021900206704721
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- Web of Science research areas
- Pathology