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High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents
Journal article   Open access

High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents

Boris Polyak, Ilia Fishbein, Michael Chorny, Ivan Alferiev, Darryl Williams, Ben Yellen, Gary Friedman and Robert J Levy
Proceedings of the National Academy of Sciences - PNAS, v 105(2), pp 698-703
15 Jan 2008
PMID: 18182491
url
https://doi.org/10.1073/pnas.0708338105View
Published, Version of Record (VoR) Open

Abstract

nanotechnology Biological Sciences gene therapy local delivery cell therapy
A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: ( i ) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and ( ii ) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.

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Collaboration types
Domestic collaboration
Web of Science research areas
Cardiac & Cardiovascular Systems
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