Journal article
High mobility group protein HMGB2 is a critical regulator of plasmodium oocyst development
The Journal of biological chemistry, v 283(25), pp 17030-17038
20 Jun 2008
PMID: 18400754
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The sexual cycle of Plasmodium is required for transmission of malaria from mosquitoes to mammals, but how parasites induce the expression of genes required for the sexual stages is not known. We disrupted the Plasmodium yoelii gene encoding high mobility group nuclear factor hmgb2, which encodes a DNA-binding protein potentially implicated in transcriptional regulation of malaria gene expression. We investigated its function in vivo in the vertebrate and invertebrate hosts. Deltapyhmgb2 parasites develop into gametocytes but have drastic impairment of oocyst formation. A global transcriptome analysis of the Deltapyhmgb2 parasites identified approximately 30 genes whose expression is down-regulated in the Deltapyhmgb2 parasites. These genes are conserved in all malaria species, and more than 90% of these genes show a peak of mRNA expression at the gametocyte stage. Surprisingly, the transcripts coding for the Plasmodium berghei orthologues of those genes are stored and translated in the ookinete stage. Therefore, sexual stage protein expression appears to be both transcriptionally and translationally regulated with Plasmodium HMGB2 acting as an important regulator of malaria sexual stage gene expression.
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Details
- Title
- High mobility group protein HMGB2 is a critical regulator of plasmodium oocyst development
- Creators
- Mathieu Gissot - Department of Medicine and of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USALi-Min TingThomas M DalyLawrence W BergmanPhotini SinnisKami Kim
- Publication Details
- The Journal of biological chemistry, v 283(25), pp 17030-17038
- Publisher
- ASBMB Publications / Elsevier; United States
- Grant note
- R01 AI056840 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; [Retired Faculty]
- Web of Science ID
- WOS:000256720600009
- Scopus ID
- 2-s2.0-47749116467
- Other Identifier
- 991014878004904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology