Journal article
High-throughput high content quantification of HIV-1 viral infectious output
PloS one, v 21(3), e0328121
2026
PMID: 41886485
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Infection with human immunodeficiency virus (HIV-1) remains a global health issue and still drives the development of significant pathology and various comorbidities. Antiretroviral therapy (ART) can effectively suppress viral replication but is often initiated months or years after initial infection, leaving a substantial period in which viral replication progresses unchecked. While ART suppresses HIV-1 replication, it does not prohibit the development of HIV-1-associated comorbidities, highlighting a lack of understanding in the connection between replication and HIV-1-associated pathogeneses. Further, a high percentage of all HIV-1 virions produced are non-infectious, and this proportion is much higher in ART-treated individuals, showing that despite inefficient viral replication, which becomes even less efficient with ART, HIV-1 is still able to drive disease. Thus, it is critical to better define HIV-1 replication dynamics to more effectively target different stages of the viral replication cycle in distinct cell populations. Here, we show a high-content imaging reporter assay that uses modified human osteosarcoma cells expressing HIV-1 receptors (GHOST cells) which fluoresce in response to HIV-1 infection. These cells have been previously used to assess HIV-1 infectivity and tropism, but this modified assay enables rapid evaluation of large numbers of samples with consistency and replicability, while also easily integrating into existing experimental pipelines that analyze p24 secretion in collected supernatants. This also allows for direct correlation between infectivity and p24 secretion, resulting in a deeper interrogation and more robust understanding of HIV-1 infection kinetics.
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Details
- Title
- High-throughput high content quantification of HIV-1 viral infectious output
- Creators
- Teresa LuPone - Drexel UniversityAlexis Brantly - Drexel UniversityOluwatofunmi Oteju - Drexel UniversityStephanie M Matt - Drexel UniversityKaitlyn Runner - Drexel UniversityEmily A Nickoloff-Bybel - Drexel UniversityMichael R Nonnemacher - Drexel UniversityPeter J Gaskill (Corresponding Author) - Drexel University
- Publication Details
- PloS one, v 21(3), e0328121
- Publisher
- PLOS
- Number of pages
- 25
- Grant note
- National Institute of Neurological Disorders and Stroke: R01NS089435 National Institute of Mental Health: K01MH132466 National Institute on Drug Abuse: R01DA057337
This work was supported by grants from the National Institutes of Drug Abuse (R01DA057337, R61DA058501 to PJG), the National Institutes on Mental Health (K01MH132466 to SMM, T32MH079785 fellowship to ENB) and the National Institute of Neurological Disorders and Stroke (R01NS089435 to MRN). Additional funding from the Departments of Pharmacology and Physiology and Microbiology and Immunology and Pharmacology and Physiology at the Drexel University College of Medicine, and from the Clinical and Translational Research Support Core (CTRSC) of the Comprehensive NeuroHIV Center (CNHC) at Temple and Drexel University.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; College of Medicine; Pharmacology and Physiology
- Web of Science ID
- WOS:001724497200018
- Scopus ID
- 2-s2.0-105033780873
- Other Identifier
- 991022171689404721
UN Sustainable Development Goals (SDGs)
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Source: SDGs in the Output
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- Web of Science research areas
- Virology