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Highly conserved Plasmodium vivax genomes in Duffy-negative individuals from Sudan
Journal article   Open access   Peer reviewed

Highly conserved Plasmodium vivax genomes in Duffy-negative individuals from Sudan

Regan E Schroeder, Safaa Ahmed, Anthony Ford, Mohammed Elfaki, Samuel Omer Hamad, Tarig Mohamed Elfaki, Sumaia Mohamed, Emilia Manko, Taane G Clark, Susana Campino, …
Scientific reports, v 15(1), 44916
29 Dec 2025
PMID: 41461753
url
https://doi.org/10.1038/s41598-025-28797-7View
Published, Version of Record (VoR) Open

Abstract

Antigens, Protozoan - genetics Duffy Blood-Group System - genetics Erythrocytes - metabolism Erythrocytes - parasitology Genome, Protozoan Humans Malaria, Vivax - epidemiology Malaria, Vivax - genetics Malaria, Vivax - immunology Malaria, Vivax - parasitology Plasmodium vivax - genetics Protozoan Proteins - genetics Receptors, Cell Surface - genetics Sudan - epidemiology Malaria
Duffy-negatives were previously thought to be immune to Plasmodium vivax infections due to Duffy binding protein's (PvDBP1) inability to invade erythrocytes lacking Duffy antigen receptor for chemokines (DARC) expression. Nevertheless, reports of P. vivax cases are growing throughout Africa and among Duffy-negative people. Although there are alternative invasion mechanisms by P. vivax, the exact mechanisms in Duffy-negative individuals are unclear. Sudan, with a mixed Duffy-negative and Duffy-positive population, is ideal to study differences between these infections on epidemiological and genetic scales. The goal of this study was to compare Duffy-positive and Duffy-negative infections in Sudanese individuals on epidemiological and genomic scales. We collected epidemiological data and sequenced parasite genomes and found that Duffy-positive individuals had significantly higher parasitemia than Duffy-negatives. Furthermore, Duffy-positive infected P. vivax genomes were much more diverse than Duffy-negatives, across all 14 chromosomes and 44 specific erythrocyte binding gene candidates. Genes of the merozoite surface protein family account for much of the genetic diversity found. Many erythrocyte binding gene candidates are under selection pressure, both positive and negative. Finally, in DBP and RBP genes, as well as TRAg38, changes in amino acids in the binding regions to a structurally different residue could affect erythrocyte binding affinity and antigenic conformation.

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Collaboration types
Domestic collaboration
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Web of Science research areas
Infectious Diseases
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