Journal article
Highly sensitive detection of HER2 extracellular domain (ECD) in the serum of breast cancer patients by piezoelectric microcantilevers (PEMS)
Analytical chemistry (Washington), v 83(9), pp 3392-3397
01 May 2011
PMID: 21449604
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Rapid and sensitive detection of serum tumor biomarkers are needed to monitor cancer patients for disease progression. Highly sensitive piezoelectric microcantilever sensors (PEMS) offer an attractive tool for biomarker detection, however their utility in the complex environment encountered in serum has yet to be determined. As a proof of concept, we have functionalized PEMS with antibodies that specifically bind to HER2, a biomarker (antigen) that is commonly overexpressed in the blood of breast cancer patients. The function and sensitivity of these anti-HER2 PEMS biosensors was initially assessed using recombinant HER2 spiked into human serum. Their ability to detect native HER2 present in the serum of breast cancer patients was then determined. We have found that the anti-HER2 PEMS were able to accurately detect both recombinant and naturally occurring HER2 at clinically relevant levels (>2 ng/ml). This indicates that PEMS-based biosensors provide a potentially effective tool for biomarker detection.
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Details
- Title
- Highly sensitive detection of HER2 extracellular domain (ECD) in the serum of breast cancer patients by piezoelectric microcantilevers (PEMS)
- Creators
- LiNa Loo - Developmental Therapeutics Program, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111Joseph A Capobianco - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104Wei Wu - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104Xiaotong Gao - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104Wan Y Shih - School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadephia, PA 19104Wei-Heng Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104Kambiz Pourrezaei - School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadephia, PA 19104Matthew K Robinson - Developmental Therapeutics Program, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111Gregory P Adams - Developmental Therapeutics Program, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111
- Publication Details
- Analytical chemistry (Washington), v 83(9), pp 3392-3397
- Publisher
- American Chemical Society; Washington, DC
- Grant note
- R01 CA118159-05 || CA / National Cancer Institute : NCI
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000289956800027
- Scopus ID
- 2-s2.0-79955556967
- Other Identifier
- 991014877812204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical