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Hippocampal neuropeptide Y protein expression following controlled cortical impact and posttraumatic epilepsy
Journal article   Peer reviewed

Hippocampal neuropeptide Y protein expression following controlled cortical impact and posttraumatic epilepsy

Zhuopin Sun, Shiying Liu, Elena A. Kharlamov, Eric R. Miller and Kevin M. Kelly
Epilepsy & behavior, v 87
Oct 2018
DOI: https://doi.org/10.1016/j.yebeh.2018.08.002
PMID: 30146352
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Controlled cortical impact Hippocampus Neuropeptide Y Posttraumatic epilepsy Rat Traumatic brain injury
This study assessed neuropeptide Y (NPY) expression in the hippocampus after long-term survival following traumatic brain injury (TBI) induced by controlled cortical impact (CCI) with or without the development of posttraumatic epilepsy (PTE). We hypothesized that following long-term survival after CCI, the severity of tissue injury and the development of PTE would correlate with the degree of hippocampal neurodegeneration as reflected by NPY+ and neuronal nuclear antigen (NeuN)+ cell loss. Adult Sprague–Dawley rats of 2–3 months of age were lesioned in the right parietal cortex and monitored for seizure activity by video and/or video-EEG. Neuropeptide Y and NeuN immunoreactivities (IRs) were quantified by light microscopy and semiautomatic image analysis approaches for unbiased quantification. Severely injured animals, marked by extensive tissue loss in the ipsilateral neocortex and adjacent hippocampus, resulted in significantly lower NeuN+ hilar cell density and NPY+ cell loss in the contralateral Cornu Ammonis (CA)-3 and dentate hilus (DH). The degree of NPY+ cell loss was more severe in CCI-injured animals with PTE than those animals that did not develop PTE. Mildly injured animals demonstrated no significant change of NPY expression compared with control animals. Our findings of long-term alterations of NPY expression in the hippocampus of severely brain-injured animals can provide important insights into the cellular and molecular consequences of severe TBI and posttraumatic epileptogenesis. •CCI-induced TBI in rats caused mild or severe tissue loss after long-term survival.•Rats with mild tissue loss had hippocampal NPY expression similar to controls.•With severe injury, the ipsilateral hippocampus was destroyed or markedly deformed.•Contralateral hippocampal NPY expression decreased with increased CCI severity.•NPY+ cell loss was more pronounced in PTE vs. non-PTE animals.

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12 citations in Web of Science
11 citations in Scopus

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Collaboration types
Domestic collaboration
Web of Science research areas
Behavioral Sciences
Clinical Neurology
Psychiatry
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