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Horizontal transfer of bacterial symbionts: heritability and fitness effects in a novel aphid host
Journal article   Open access   Peer reviewed

Horizontal transfer of bacterial symbionts: heritability and fitness effects in a novel aphid host

Jacob A Russell and Nancy A Moran
Applied and environmental microbiology, v 71(12), pp 7987-7994
Dec 2005
PMID: 16332777
url
https://doi.org/10.1128/AEM.71.12.7987-7994.2005View
Published, Version of Record (VoR) Open

Abstract

Gammaproteobacteria - isolation & purification Aphids - microbiology Animals DNA, Bacterial - genetics Aphids - classification Base Sequence Polymerase Chain Reaction Female Gammaproteobacteria - genetics DNA Primers Gene Transfer, Horizontal - genetics Symbiosis - genetics
Members of several bacterial lineages are known only as symbionts of insects and move among hosts through maternal transmission. Such vertical transfer promotes strong fidelity within these associations, favoring the evolution of microbially mediated effects that improve host fitness. However, phylogenetic evidence indicates occasional horizontal transfer among different insect species, suggesting that some microbial symbionts retain a generalized ability to infect multiple hosts. Here we examine the abilities of three vertically transmitted bacteria from the Gammaproteobacteria to infect and spread within a novel host species, the pea aphid, Acyrthosiphon pisum. Using microinjection, we transferred symbionts from three species of natural aphid hosts into a common host background, comparing transmission efficiencies between novel symbionts and those naturally infecting A. pisum. We also examined the fitness effects of two novel symbionts to determine whether they should persist under natural selection acting at the host level. Our results reveal that these heritable bacteria vary in their capacities to utilize A. pisum as a host. One of three novel symbionts failed to undergo efficient maternal transmission in A. pisum, and one of the two efficiently transmitted bacteria depressed aphid growth rates. Although these findings reveal that negative fitness effects and low transmission efficiency can prevent the establishment of a new infection following horizontal transmission, they also indicate that some symbionts can overcome these obstacles, accounting for their widespread distributions across aphids and related insects.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biotechnology & Applied Microbiology
Microbiology
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