Journal article
Host-parasite Interactions Revealed by Plasmodium falciparum Metabolomics
Cell host & microbe, v 5(2), pp 191-199
19 Feb 2009
PMID: 19218089
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Intracellular pathogens have devised mechanisms to exploit their host cells to ensure their survival and replication. The malaria parasite
Plasmodium falciparum
relies on an exchange of metabolites with the host for proliferation. We describe the first mass spectrometry-based metabolomic analysis of the parasite throughout its 48-hour intraerythrocytic developmental cycle. Our results reveal a general modulation of metabolite levels by the parasite, with numerous metabolites varying in phase with the developmental cycle. Others differed from uninfected cells irrespective of the developmental stage. Among these was extracellular arginine, which was specifically converted to ornithine by the parasite. To identify the biochemical basis for this effect, we disrupted the plasmodium arginase gene in the rodent malaria model
P. berghei
. These parasites were viable but did not convert arginine to ornithine. Our results suggest that systemic arginine depletion by the parasite may be a factor in human malarial hypoargininemia associated with cerebral malaria pathogenesis.
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Details
- Title
- Host-parasite Interactions Revealed by Plasmodium falciparum Metabolomics
- Creators
- Kellen L Olszewski - Department of Molecular Biology & Lewis-Sigler Institute for Integrative Genomics Princeton University, Princeton, NJ 08544, USAJoanne M Morrisey - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USADaniel Wilinski - Department of Molecular Biology & Lewis-Sigler Institute for Integrative Genomics Princeton University, Princeton, NJ 08544, USAJames M Burns - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USAAkhil B Vaidya - Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USAJoshua D Rabinowitz - Department of Chemistry & Lewis-Sigler Institute for Integrative Genomics Princeton University, Princeton, NJ 08544Manuel Llinás - Department of Molecular Biology & Lewis-Sigler Institute for Integrative Genomics Princeton University, Princeton, NJ 08544, USA
- Publication Details
- Cell host & microbe, v 5(2), pp 191-199
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000263814000010
- Scopus ID
- 2-s2.0-60649112444
- Other Identifier
- 991014877943504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology
- Parasitology
- Virology