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Journal article
Hsa-miR-605 regulates the proinflammatory chemokine CXCL5 in complex regional pain syndrome
Biomedicine & pharmacotherapy, v 140, pp 111788-111788
01 Aug 2021
PMID: 34062414
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Complex regional pain syndrome (CRPS) is a chronic pain condition characterized by inflammation and debilitating pain. CRPS patients with pain refractory to more conventional analgesics can be treated with subanesthetic doses of ketamine. Our previous studies found that poor responders to ketamine had a 22-fold downregulation of the miRNA hsa-miR-605 in blood prior to ketamine treatment. Hence, we sought to investigate the functional significance of miR-605 downregulation and its impact on target gene expression, as investigating target mRNAs of differentially expressed miRNAs can provide important insights on aberrant gene expression that may contribute to disease etiology. Using a bioinformatics prediction, we identified that miR-605 can target the proinflammatory chemokine CXCL5, which plays a role in leukocyte recruitment and activation. We hypothesized that downregulation of miR-605 in poor responders to ketamine could increase CXCL5 expression and thereby contribute to inflammation in these patients. We confirmed that miR-605 regulates CXCL5 by using a miRNA mimic and inhibitor in human primary endothelial cells. Inhibition of miR-605 increased CXCL5 secretion and migration of human monocytic cells, thereby demonstrating a functional impact of miR-605 on chemotaxis. Additionally, CXCL5 mRNA was upregulated in whole blood from poor responders to ketamine, and CXCL5 protein was increased in plasma from CRPS patients. Thus, our studies suggest that miR-605 regulation of CXCL5 can regulate inflammation.
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Details
- Title
- Hsa-miR-605 regulates the proinflammatory chemokine CXCL5 in complex regional pain syndrome
- Creators
- Richa Pande - Drexel UniversityAyush Parikh - Drexel UniversityBotros Shenoda - Drexel UniversitySujay Ramanathan - Drexel UniversityGuillermo M. Alexander - Drexel UniversityRobert J. Schwartzman - Drexel UniversitySeena K. Ajit - Drexel University
- Publication Details
- Biomedicine & pharmacotherapy, v 140, pp 111788-111788
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- R01NS102836 / NIH NINDS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology; [Retired Faculty]; Neurology
- Web of Science ID
- WOS:000670119700001
- Scopus ID
- 2-s2.0-85107135817
- Other Identifier
- 991019168895204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Medicine, Research & Experimental
- Pharmacology & Pharmacy