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Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex
Journal article   Open access   Peer reviewed

Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex

Seiji Matsumoto, Keiko Ogino, Eishi Noguchi, Paul Russell and Hisao Masai
The Journal of biological chemistry, v 280(52), pp 42536-42542
30 Dec 2005
DOI: https://doi.org/10.1074/jbc.M510575200
PMID: 16263721
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1074/jbc.M510575200View
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Abstract

Protein Kinases - metabolism Hydroxyurea - pharmacology Protein Kinases - genetics Temperature Immunoprecipitation Alkylating Agents - pharmacology DNA, Single-Stranded - genetics DNA-Binding Proteins - metabolism Schizosaccharomyces - genetics S Phase Escherichia coli - metabolism Schizosaccharomyces pombe Proteins - metabolism Methyl Methanesulfonate - chemistry Schizosaccharomyces pombe Proteins - physiology Protein-Serine-Threonine Kinases - metabolism Schizosaccharomyces pombe Proteins - genetics Chromosomal Proteins, Non-Histone - metabolism Protein-Serine-Threonine Kinases - physiology Cell Cycle Proteins - metabolism Schizosaccharomyces - physiology DNA Replication DNA - metabolism Transcription Factors - genetics DNA-Binding Proteins - genetics Plasmids - metabolism Chromosomal Proteins, Non-Histone - genetics Transcription Factors - metabolism Two-Hybrid System Techniques Animals Cell Cycle DNA Repair Alleles Saccharomyces cerevisiae Proteins - metabolism Protein Binding Bacterial Proteins - metabolism Mice Models, Genetic Saccharomyces cerevisiae Proteins - physiology Mutation Cell Cycle Proteins - physiology Luminescent Proteins - metabolism
The protein kinase Hsk1 is essential for DNA replication in Schizosaccharomyces pombe. It associates with Dfp1/Him1 to form an active complex equivalent to the Cdc7-Dbf4 protein kinase in Saccharomyces cerevisiae. Swi1 and Swi3 are subunits of the replication fork protection complex in S. pombe that is homologous to the Tof1-Csm3 complex in S. cerevisiae. The fork protection complex helps to preserve the integrity of stalled replication forks and is important for activation of the checkpoint protein kinase Cds1 in response to fork arrest. Here we describe physical and genetic interactions involving Swi1 and Hsk1-Dfp1/Him1. Dfp1/Him1 was identified in a yeast two-hybrid screen with Swi1. Hsk1 and Dfp1/Him1 both co-immunoprecipitate with Swi1. Swi1 is required for growth of a temperature-sensitive hsk1 (hsk1ts) mutant at its semi-permissive temperature. Hsk1ts cells accumulate Rad22 (Rad52 homologue) DNA repair foci at the permissive temperature, as previously observed in swi1 cells, indicating that abnormal single-stranded DNA regions form near the replication fork in hsk1ts cells. hsk1ts cells were also unable to properly delay S-phase progression in the presence of a DNA alkylating agent and were partially defective in mating type switching. These data suggest that Hsk1-Dfp1/Him1 and Swi1-Swi3 complexes have interrelated roles in stabilization of arrested replication forks.

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Web of Science research areas
Biochemistry & Molecular Biology
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