Journal article
Human Bladder Uroepithelial Cells Synergize with Monocytes to Promote IL-10 Synthesis and Other Cytokine Responses to Uropathogenic Escherichia coli
PloS one, v 8(10), pp e78013-e78013
14 Oct 2013
PMID: 24155979
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Urinary tract infections are a major source of morbidity for women and the elderly, with Uropathogenic Escherichia coli (UPEC) being the most prevalent causative pathogen. Studies in recent years have defined a key anti-inflammatory role for Interleukin-10 (IL-10) in urinary tract infection mediated by UPEC and other uropathogens. We investigated the nature of the IL-10-producing interactions between UPEC and host cells by utilising a novel coculture model that incorporated lymphocytes, mononuclear and uroepithelial cells in histotypic proportions. This coculture model demonstrated synergistic IL-10 production effects between monocytes and uroepithelial cells following infection with UPEC. Membrane inserts were used to separate the monocyte and uroepithelial cell types during infection and revealed two synergistic IL-10 production effects based on contact-dependent and soluble interactions. Analysis of a comprehensive set of immunologically relevant biomarkers in monocyte-uroepithelial cell co-cultures highlighted that multiple cytokine, chemokine and signalling factors were also produced in a synergistic or antagonistic fashion. These results demonstrate that IL-10 responses to UPEC occur via multiple interactions between several cells types, implying a complex role for infection-related IL-10 during UTI. Development and application of the co-culture model described in this study is thus useful to define the degree of contact dependency of biomarker production to UPEC, and highlights the relevance of histotypic co-cultures in studying complex host-pathogen interactions.
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Details
- Title
- Human Bladder Uroepithelial Cells Synergize with Monocytes to Promote IL-10 Synthesis and Other Cytokine Responses to Uropathogenic Escherichia coli
- Creators
- Benjamin L. Duell - Griffith UniversityAlison J. Carey - Griffith UniversitySamantha J. Dando - Griffith UniversityMark A. Schembri - The University of QueenslandGlen C. Ulett - Griffith University
- Publication Details
- PloS one, v 8(10), pp e78013-e78013
- Publisher
- Public Library Science
- Number of pages
- 14
- Grant note
- Griffith University Australian National Health and Medical Research Council (NHMRC); National Health and Medical Research Council (NHMRC) of Australia NHMRC Peter Doherty Australian Biomedical Fellowship; National Health and Medical Research Council (NHMRC) of Australia Australian Research Council Future Fellowship; Australian Research Council
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine; Drexel University
- Web of Science ID
- WOS:000325887300113
- Scopus ID
- 2-s2.0-84885417061
- Other Identifier
- 991020100079504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology